Macrophage Participation in the Degradation and Remodeling of Extracellular Matrix Scaffolds

Biologic scaffolds composed of extracellular matrix (ECM) are widely used to facilitate remodeling and reconstruction of a variety of tissues in both preclinical animal studies and human clinical applications. The mechanisms by which such scaffolds influence the host tissue response are only partial...

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Bibliographic Details
Published inTissue engineering. Part A Vol. 15; no. 7; pp. 1687 - 1694
Main Authors Valentin, Jolene E., Stewart-Akers, Ann M., Gilbert, Thomas W., Badylak, Stephen F.
Format Journal Article
LanguageEnglish
Published United States Mary Ann Liebert, Inc 01.07.2009
Subjects
Animals
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
Carbodiimides - pharmacology
Cellular biology
Cross-Linking Reagents - pharmacology
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Fluorescent Antibody Technique
Immunohistochemistry
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Macrophages - metabolism
Male
Original
Original Articles
Postoperative Care
Prosthesis Implantation
Rats
Rodents
Sus scrofa
Tissue engineering
Tissue Scaffolds
Transplantation, Autologous
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Summary:Biologic scaffolds composed of extracellular matrix (ECM) are widely used to facilitate remodeling and reconstruction of a variety of tissues in both preclinical animal studies and human clinical applications. The mechanisms by which such scaffolds influence the host tissue response are only partially understood, but it is logical that the mononuclear macrophage cell population plays a central role. The present study evaluated the role of macrophages that derive from peripheral blood in the degradation of ECM scaffolds. An established rat body wall reconstruction model was used to evaluate the degradation of carbodiimide (CDI)–crosslinked scaffolds composed of porcine small intestinal submucosa (SIS), noncrosslinked SIS, and autologous body wall. To assess the role of circulating macrophages in the degradation process, the degradation of each scaffold was assessed with and without macrophage depletion caused by administration of clodronate-containing liposomes. Results showed that peripheral blood monocytes are required for the early and rapid degradation of both SIS scaffolds and autologous body wall, and that CDI crosslinked SIS is resistant to macrophage-mediated degradation.
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ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2008.0419