Fluorometric Analysis of Intracellular Sodium Concentrations in Isolated Retinal Horizontal Cells

• Published in: The Biological bulletin (Lancaster) Vol. 195; no. 2; pp. 204 - 205
• Main Authors: Andersen, K. A., Malchow, R. P.
• Format: Journal Article
• Language: English
• Published: United States Marine Biological Laboratory 01.10.1998; University of Chicago Press
• Subjects: Animals; Anti-Arrhythmia Agents - pharmacology; Behavior and Neurobiology; Benzofurans - chemistry; Biological transport; Calibration; Cells; Dyes; Electrodes; Ethers, Cyclic - chemistry; Fish; Fluorescence; Fluorescent Dyes - chemistry; Fluorometry - methods; Image Processing, Computer-Assisted; Imaging; Intracellular Fluid - chemistry; Intracellular Fluid - drug effects; Intracellular Fluid - metabolism; Marine; Neurons; Neurons, Afferent - chemistry; Neurons, Afferent - drug effects; Physiological aspects; Quinidine; Quinidine - pharmacology; Raja erinacea; Raja ocellata; Retina; Retina - chemistry; Retina - cytology; Retina - metabolism; Skates (Fish); Sodium; Sodium (Nutrient); Sodium - analysis; Sodium - metabolism; Sodium in the body
• ISSN: 0006-3185; 1939-8697
• DOI: 10.2307/1542840

Sodium-binding benzofuran isophthalate, a sodium-sensitive fluorescent indicator, was used in the fluorometric measurement of intracellular sodium concentration in the horizontal cells isolated from the retina of the skate to determine the effect of quinidine on intracellular sodium levels. Results reveal that fluorescent indicators are useful in the measurement of intracellular sodium levels. Further, quinidine promotes an increase in the intracellular sodium concentration in skate retinal horizontal cells.