Distribution of cells expressing vomeronasal receptors in the olfactory organ of turtles

• Published in: Journal of Veterinary Medical Science Vol. 82; no. 8; pp. 1068 - 1079
• Main Authors: ABDALI, Sayed Sharif, NAKAMUTA, Shoko, YAMAMOTO, Yoshio, NAKAMUTA, Nobuaki
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2020; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Anatomy; Animals; Female; Gene Expression; Genes; in situ hybridization; Male; Odorant receptors; Olfaction; Olfactory epithelium; Olfactory Mucosa - cytology; Olfactory Mucosa - metabolism; olfactory organ; Olfactory organs; Olfactory receptor neurons; Olfactory Receptor Neurons - metabolism; Receptors, Odorant - genetics; Receptors, Odorant - metabolism; Reptiles & amphibians; Signal transduction; Smell; transient receptor potential cation channel subfamily C member 2; turtle; Turtles; Ultrastructure; Vomeronasal organ; Vomeronasal Organ - cytology; Vomeronasal Organ - metabolism; vomeronasal receptors; Vomeronasal system; vomeronasal receptors; turtle; transient receptor potential cation channel subfamily C member 2; in situ hybridization; olfactory organ
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.20-0207

Generally, the olfactory organ of vertebrates consists of the olfactory epithelium (OE) and the vomeronasal organ (VNO). The OE contains ciliated olfactory receptor neurons (ORNs), while the VNO contains microvillous ORNs. The ORNs in the OE express odorant receptors (ORs), while those in the VNO express type 1 and type 2 vomeronasal receptors (V1Rs and V2Rs). In turtles, the olfactory organ consists of the upper (UCE) and lower chamber epithelia (LCE). The UCE contains ciliated ORNs, while the LCE contains microvillous ORNs. Here we investigated the distribution of cells expressing vomeronasal receptors in the olfactory organ of turtles. The turtle vomeronasal receptors were encoded by two V1R genes and two V2R genes. Among them, V2R1 and V2R26 were mainly expressed in the LCE, while V1R3 was expressed both in the UCE and LCE. Notably, vomeronasal receptors were expressed by a limited number of ORNs, which was confirmed by the expression of the gene encoding TRPC2, an ion channel involved in the signal transduction of vomeronasal receptors. Furthermore, expression of ORs by the majority of ORNs was suggested by the expression of the gene encoding CNGA2, an ion channel involved in the signal transduction of ORs. Thus, olfaction of turtle seems to be mediated mainly by the ORs rather than the vomeronasal receptors. More importantly, the relationship between the fine structure of ORNs and the expression of olfactory receptors are not conserved among turtles and other vertebrates.