Protective effect of Rhus coriaria fruit extracts against hydrogen peroxide-induced oxidative stress in muscle progenitors and zebrafish embryos

• Published in: PeerJ (San Francisco, CA) Vol. 5; p. e4144
• Main Authors: Najjar, Fadia, Rizk, Francine, Carnac, Gilles, Nassar, Rim, Jabak, Sara, Sobolev, Anatoly Petrovich, Bou Saada, Yara, El Sabban, Marwan, Hamade, Aline
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 12.12.2017; PeerJ, Inc; PeerJ; PeerJ Inc
• Subjects: Acetic acid; Aging; Analysis; Antioxidants; Atrophy; Biology; Catalase; Cell adhesion; Cell Biology; Cell cycle; Cyclin-dependent kinases; Danio rerio; Embryonic development; Embryos; Ethanol; Ethyl acetate; Flow cytometry; Free radicals; Gallic acid; Gene expression; Gene flow; Hydrogen peroxide; Life Sciences; Metabolism; Muscle contraction; Musculoskeletal system; Myoblast (LHCN-M2); Myoblasts; NMR; Nuclear magnetic resonance; Nutrition research; Oxidative stress; Physiological aspects; Physiology; Properties; Rhus coriaria; Rodents; Skeletal muscle; Stem cells; Studies; Sumac Rhus coriaria extract; Superoxide dismutase; Translational Medicine; Zebrafish; Zebrafish embryos; Antioxidants; Aging; Zebrafish embryos; NMR; Sumac Rhus coriaria extract; Myoblast (LHCN-M2)
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.4144

Oxidative stress is involved in normal and pathological functioning of skeletal muscle. Protection of myoblasts from oxidative stress may improve muscle contraction and delay aging. Here we studied the effect of sumac fruit extract on human myoblasts and zebrafish embryos in conditions of hydrogen peroxide-induced oxidative stress. Crude ethanolic 70% extract (CE) and its fractions was obtained from sumac fruits. The composition of sumac ethyl acetate EtOAc fraction was studied by H NMR. The viability of human myoblasts treated with CE and the EtOAc fraction was determined by trypan blue exclusion test. Oxidative stress, cell cycle and adhesion were analyzed by flow cytometry and microscopy. Gene expression was analyzed by qPCR. The EtOAc fraction (IC 2.57 µg/mL) had the highest antioxidant activity and exhibited the best protective effect against hydrogen peroxide-induced oxidative stress. It also restored cell adhesion. This effect was mediated by superoxide dismutase 2 and catalase. Pre-treatment of zebrafish embryos with low concentrations of the EtOAc fraction protected them from hydrogen peroxide-induced death . H NMR analysis revealed the presence of gallic acid in this fraction. extracts inhibited or slowed down the progress of skeletal muscle atrophy by decreasing oxidative stress via superoxide dismutase 2 and catalase-dependent mechanisms.