Effects of between and within Herd Moves on Elephant Endotheliotropic Herpesvirus (EEHV) Recrudescence and Shedding in Captive Asian Elephants (Elephas maximus)

• Published in: Viruses Vol. 14; no. 2; p. 229
• Main Authors: Titus, Sanna Eriksson, Patterson, Stuart, Prince-Wright, Joanna, Dastjerdi, Akbar, Molenaar, Fieke Marije
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.01.2022; MDPI
• Subjects: Adults; Animals; Animals, Zoo - physiology; Animals, Zoo - virology; Aquariums; Behavior, Animal; bulls; carrier state; cows; death; DNA; DNA, Viral - genetics; elephant endotheliotropic herpesvirus; Elephantid betaherpesvirus 1; Elephants; Elephants - physiology; Elephants - virology; Elephas maximus; Epidemiology; Exocrine glands; Fatalities; Female; Hemorrhagic disease; herds; Herpes viruses; Herpesviridae - classification; Herpesviridae - genetics; Herpesviridae - isolation & purification; Herpesviridae - physiology; Herpesviridae Infections - transmission; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Infections; Latent infection; Longitudinal Studies; Male; management; Medical screening; qPCR; quantitative polymerase chain reaction; recrudescence; risk; Sexual Behavior, Animal; Social change; Social interactions; Viral Load; Viral Proteins - genetics; Viral Proteins - metabolism; Virus Shedding; Zoo animals; zoos; United Kingdom > UK; North America; Europe; Germany; elephant endotheliotropic herpesvirus; recrudescence; qPCR; management; social change; Elephas maximus
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v14020229

Haemorrhagic disease associated with elephant endotheliotropic herpesvirus (Elephantid herpesvirus, EEHV) infections is the leading cause of death for Asian elephant (Elephas maximus) calves. This study assessed the effect of captive herd management on EEHV shedding, as evidence of latent infection reactivation, focusing on: (1) the influence of social change on the odds of recrudescence; (2) the respective effects of between and within herd moves; and (3) characteristics of recrudescent viral shedding. Trunk and conjunctival swabs (n = 165) were obtained from six elephants at an EAZA-accredited zoo, collected during a period of social stability, and at times of social change. Longitudinal sampling took place at times of moving two bulls out of the collection and one new bull into an adjacent enclosure to the cow herd (between herd moves), and during a period of mixing this new bull with the cow herd to facilitate mating (within herd moves). Quantitative PCR was employed to detect EEHV 1a/b, 4a/b, and EF–1–α (housekeeping gene). Generalised estimating equations determined EEHV recrudescence odds ratios (OR) and relative viral DNA load. Sixteen EEHV 1a/b shedding events occurred, but no EEHV 4a/b was detected. All management-derived social changes promoted recrudescence (social change OR = 3.27, 95% CI = 0.412–26, p = 0.262; and between herd moves OR = 1.6, 95% CI = 0.178−14.4, p = 0.675), though within herd movements posed the most significant increase of EEHV reactivation odds (OR = 6.86, 95% CI = 0.823−57.1, p = 0.075) and demonstrated the strongest relative influence (post hoc Tukey test p = 0.0425). Shedding onset and magnitude ranged from six to 54 days and from 3.59 to 11.09 ΔCts. Differing challenges are associated with between and within herd movements, which can promote recrudescence and should be considered an exposure risk to naïve elephants.