Pediocin PA-1 production during repeated-cycle batch culture of immobilized Pediococcus acidilactici UL5 cells

• Published in: Journal of bioscience and bioengineering Vol. 105; no. 5; pp. 513 - 517
• Main Authors: Naghmouchi, Karim, Fliss, Ismail, Drider, Djamel, Lacroix, Christophe
• Format: Journal Article
• Language: English
• Published: Amsterdarm Elsevier B.V 01.05.2008; Osaka, Japan: Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands: Distributed outside Japan by Elsevier Science; Elsevier Science; Elsevier Limited
• Subjects: Bacteriocins - isolation & purification; Bacteriocins - metabolism; batch fermentation; Biological and medical sciences; Bioreactors - microbiology; Biotechnology; Cell Culture Techniques - methods; Cells, Immobilized; fermentation; Fundamental and applied biological sciences. Psychology; immobilized cells; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; pediocin PA-1; pediocin PA-1/AcH; Pediocins; Pediococcus - growth & development; Pediococcus - metabolism; Pediococcus acidilactici; production; protein synthesis; repeated-cycle; repeated-cycle batch culture; fermentation; repeated-cycle; pediocin PA-1/AcH; production; Streptococcaceae; Lactic acid bacteria; Batchwise; Bacteriocin; Production; Bacteria; Micrococcales; Fermentation; Pediococcus acidilactici; Culture
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.105.513

Pediocin PA-1 production by Pediococcus acidilactici UL5 cells immobilized in κ-carrageenan/locust bean gum gel beads was studied during repeated-cycle batch (RCB) culture with pH control in Man Rogosa and Sharpe (MRS) broth supplemented with 1% glucose and whey permeate (SWP) medium. The pediocin PA-1 production by free P. acidilactici cells pH-controlled batch culture has reached 2048 and 4096 AU ml −1 after 11 and 12 h of incubation, with volumetric productivities of 187 and 342 AU ml −1 h −1 in SWP and MRS media, respectively. In RCB culture, immobilized cells reached a maximum concentration of 7.3±0.2×10 10 and 4.3±0.9×10 10 cfu g −1 of beads in MRS and SWP media, respectively. The maximum pediocin PA-1 activity obtained during RCB fermentation was 4096 AU ml −1; it was attained after only 0.75 and 2 h of incubation in MRS and SWP media, respectively. The corresponding volumetric productivities were 5461 and 2048 AU ml −1 h −1. Pediocin PA-1 production in the RCB culture was highly stable over 12 fermentation cycles carried out over 3 d in SWP media.