Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serum and Bone in Rat Model of Glucocorticoid-Induced Osteoporosis

• Published in: Molecules (Basel, Switzerland) Vol. 21; no. 11; p. 1523
• Main Authors: Mohamad Asri, Siti Fadziyah, Mohd Ramli, Elvy Suhana, Soelaiman, Ima Nirwana, Mat Noh, Muhamad Alfakry, Abdul Rashid, Abdul Hamid, Suhaimi, Farihah
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 15.11.2016; MDPI AG
• Subjects: 11-beta-Hydroxysteroid Dehydrogenase Type 1 - blood; 11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism; 11β-hydroxysteroid dehydrogenase; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Antioxidants - chemistry; Antioxidants - pharmacology; Biomarkers; Body Weight - drug effects; Bone and Bones - drug effects; Bone and Bones - metabolism; Corticosterone - blood; Corticosterone - metabolism; Disease Models, Animal; glucocorticoid; Glucocorticoids - adverse effects; osteoporosis; Osteoporosis - blood; Osteoporosis - drug therapy; Osteoporosis - etiology; Osteoporosis - metabolism; Piper; Piper - chemistry; Piper sarmentosum; Plant Extracts - chemistry; Plant Extracts - pharmacology; Rats; 11β-hydroxysteroid dehydrogenase; Piper sarmentosum; glucocorticoid; osteoporosis
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules21111523

Glucocorticoid-induced osteoporosis is one of the common causes of secondary osteoporosis. ( ) extract possesses antioxidant and anti-inflammatory activities. In this study, we determined the correlation between the effects of leaf water extract with the regulation of 11β-hydroxysteroid dehydrogenase (HSD) type 1 enzyme activity in serum and bone of glucocorticoid-induced osteoporotic rats. Twenty-four Sprague-Dawley rats were grouped into following: G1: sham-operated group administered with intramuscular vehicle olive oil and vehicle normal saline orally; G2: adrenalectomized (adrx) control group given intramuscular dexamethasone (120 μg/kg/day) and vehicle normal saline orally; G3: adrx group given intramuscular dexamethasone (120 μg/kg/day) and water extract of (125 mg/kg/day) orally. After two months, the femur and serum were taken for ELISA analysis. Results showed that leaf water extract significantly reduced the femur corticosterone concentration ( < 0.05). This suggests that leaf water extract was able to prevent bone loss due to long-term glucocorticoid therapy by acting locally on the bone cells by increasing the dehydrogenase action of 11β-HSD type 1. Thus, may have the potential to be used as an alternative medicine against osteoporosis and osteoporotic fracture in patients on long-term glucocorticoid treatment.