Inhibitory Effects of Isorhamnetin-3-O-β-D-glucoside from Salicornia herbacea on Rat Lens Aldose Reductase and Sorbitol Accumulation in Streptozotocin-Induced Diabetic Rat Tissues

• Published in: Biological & pharmaceutical bulletin Vol. 28; no. 5; pp. 916 - 918
• Main Authors: Lee, Yeon Sil, Lee, Sanghyun, Lee, Hye Seung, Kim, Bak-Kwang, Ohuchi, Kazuo, Shin, Kuk Hyun
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01.05.2005
• Subjects: Aldehyde Reductase - antagonists & inhibitors; Aldehyde Reductase - metabolism; Animals; Chenopodiaceae; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Enzyme Inhibitors - therapeutic use; Flavonols - isolation & purification; Flavonols - pharmacology; Flavonols - therapeutic use; Glucosides - isolation & purification; Glucosides - pharmacology; Glucosides - therapeutic use; isorhamnetin-3-O-β-D-glucoside; Lens, Crystalline - drug effects; Lens, Crystalline - metabolism; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Quercetin - analogs & derivatives; rat lens aldose reductase; Rats; Rats, Sprague-Dawley; Salicornia herbacea; serum glucose concentration; Sorbitol - antagonists & inhibitors; Sorbitol - metabolism; sorbitol accumulation; Tissue Distribution - drug effects; Tissue Distribution - physiology
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.28.916

The inhibitory effects of compounds from Salicornia herbacea (Chenopodiaceae) on rat lens aldose reductase (RLAR) and sorbitol accumulation in streptozotocin-induced diabetic rat tissues were investigated. The various fractions from the MeOH extract of S. herbacea were tested for their effects on RLAR in vitro. Among them, the EtOAc fraction was found to exhibit a potent RLAR inhibition (IC50=0.75 μg/ml), from which an active principle as a potent AR inhibitor was isolated and its chemical structure was elucidated as isorhamnetin-3-O-β-D-glucoside (1) by spectral analysis. Compound 1 exhibited a potent RLAR inhibition in vitro, its IC50 being 1.4 μM. Compound 1, when administered orally at 25 mg/kg in streptozotocin (STZ)-induced diabetic rats, caused not only a significant inhibition of serum glucose concentration but also sorbitol accumulation in the lenses, red blood cells (RBC), and sciatic nerves. These results indicate that compound 1 from S. herbacea is a leading compound for further study as a new drug for the prevention and/or treatment of diabetes and its complications.