Functional characterization of two cold shock domain proteins from Oryza sativa

• Published in: Plant, cell and environment Vol. 31; no. 7; pp. 995 - 1006
• Main Authors: CHAIKAM, VIJAY, KARLSON, DALE
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.07.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Base Sequence; Biological and medical sciences; Blotting, Western; cold acclimation; Cold Temperature; DNA Primers; DNA, Complementary; Enzymes; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; gene regulation; Genetic Complementation Test; low‐temperature stress; Metabolism; Oryza - genetics; Oryza - physiology; Oryza sativa; plant development; Plant physiology and development; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Proteins - physiology; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Triticum aestivum; Monocotyledones; Cold; low- temperature stress; cold acclimation; Stress; Oryza sativa; plant development; Gene; Gramineae; gene regulation; Angiospermae; Development; Spermatophyta; Low temperature
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/j.1365-3040.2008.01811.x

Two novel rice cold shock domain (CSD) proteins were cloned and characterized under different stress treatments and during various stages of development. OsCSP1 and OsCSP2 (Oryza sativa CSD protein) encode putative proteins consisting of an N-terminal CSD and glycine-rich regions that are interspersed by 4 and 2 CX₂CX₄HX₄C (CCHC) retroviral-like zinc fingers, respectively. In vivo functional analysis confirmed that OsCSPs can complement a cold-sensitive bacterial strain which lacks four endogenous cold shock proteins. In vitro ssDNA binding assays determined that recombinant OsCSPs are capable of functioning as nucleic acid-binding proteins. Both OsCSP transcripts are transiently up-regulated in response to low-temperature stress and rapidly return to a basal level of gene expression. Protein blot analysis determined that OsCSPs are maintained at a constant level subsequent to a cold treatment lasting over a period of several days. Both the transcript and protein data are in sharp contrast to those previously obtained for winter wheat WCSP1. A time-coursed study through various stages of rice development confirmed that both OsCSP proteins and transcripts are highly accumulated in reproductive tissues and tissues which exhibit meristematic activity.