海洋胶红酵母Y2产类胡萝卜素发酵促进剂的研究

研究了几种添加物对海洋胶红酵母（Rhodotorulamucilaginosa）Y2发酵产类胡萝卜素的促进作用。在摇瓶条件下，发酵培养基中分别添加不同量的番茄汁、花生油、大豆油、玉米浆、谷氨酸钠和生物素，培养72h后取样分别测定类胡萝卜素产量及生物量。结果显示，当分别添加2mL·L-1番茄汁、1．5mL·L-1花生油和3mL·L-1玉米浆时，类胡萝卜素产量比对照组提高14．2％、30．3％和21．3％；谷氨酸钠和生物素会抑制海洋胶红酵母Y2类胡萝卜素的合成。因此，番茄汁、花生油和玉米浆是较为经济适用的海洋胶红酵母Y2产类胡萝卜素发酵促进剂，具有广阔的开发应用潜力。...

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Published in南方水产科学 Vol. 9; no. 2; pp. 45 - 49
Main Author 杨铿 陈永青 梁晓华 杨莺莺 佘妙虹
Format Journal Article
LanguageChinese
Published 中国水产科学研究院南海水产研究所,广东广州,510300 2013
Subjects
发酵促进剂
海洋胶红酵母Y2
生物量
类胡萝卜素
发酵促进剂
类胡萝卜素
生物量
海洋胶红酵母Y2
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ISSN2095-0780
DOI10.3969/j.issn.2095-0780.2013.02.008

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Summary:研究了几种添加物对海洋胶红酵母（Rhodotorulamucilaginosa）Y2发酵产类胡萝卜素的促进作用。在摇瓶条件下，发酵培养基中分别添加不同量的番茄汁、花生油、大豆油、玉米浆、谷氨酸钠和生物素，培养72h后取样分别测定类胡萝卜素产量及生物量。结果显示，当分别添加2mL·L-1番茄汁、1．5mL·L-1花生油和3mL·L-1玉米浆时，类胡萝卜素产量比对照组提高14．2％、30．3％和21．3％；谷氨酸钠和生物素会抑制海洋胶红酵母Y2类胡萝卜素的合成。因此，番茄汁、花生油和玉米浆是较为经济适用的海洋胶红酵母Y2产类胡萝卜素发酵促进剂，具有广阔的开发应用潜力。
Bibliography:YANG Keng, CHEN Yongqing, LIANG Xiaohua, YANG Yingying, SHE Miaohong （South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China）
We studied the effects of several additives on the yield of carotenoid fermented by Rhodotorula mucilaginosa. In the shake flask condition, different concentrations of tomato juice, peanut oil, soybean oil, corn steep liquor, sodium glutamate and biotin were added to the fermentation medium respectively, and the carotened yields as well as R. mucilaginosa biomass were measured after 72 h. The results show that when 2 mL＇L-1 tomato juice, 1.5 mL-L-lpeanut oil, and 3 mL＇L-1 corn steep liquor are added, the yields of carotenoids are increased by 14. 2%, 30. 3% and 21.3% respectively. However, sodium glutamate and biotin may inhibit the synthe- sis of carotenoid by R. mucilaginosa. Therefore, tomato juice, peanut oil and corn steep liquor are good promoter for carotenoid fermen- tation by R. mucilaginosa, which may have great potential for
ISSN:2095-0780
DOI:10.3969/j.issn.2095-0780.2013.02.008