高通量便携式荧光检测装置的研制与应用
基于一字线激光模组，设计并构建了高通量便携式荧光检测装置，因其无需传统微孔板荧光仪所要求的专业精密调节的复杂光路系统，具有结构简单、小巧轻便、成本低廉与多通道的特点。结合自行研制的图像处理软件，对96孔微孔板样品的检测与数据处理可在10min内完成，实现了溶液的高通量定量检测。检测罗丹明B的灵敏度为（2．80±0．15）nmol／L。应用本装置检测水溶液中H矿，检出限为7．7μg/L；检测自来水样品中Hg^2＋的加标回收率为99．9％～102．4％，RSD为1．5％～2．6％；检测湖水样品中H矿的加标回收率为96．2％～98．9％，RSD为1．6％～2．5％。...
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| Published in | 分析化学 Vol. 41; no. 10; pp. 1620 - 1624 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
厦门大学化学化工学院化学系,厦门,361005
2013
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2013.30013 |
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| Abstract | 基于一字线激光模组，设计并构建了高通量便携式荧光检测装置，因其无需传统微孔板荧光仪所要求的专业精密调节的复杂光路系统，具有结构简单、小巧轻便、成本低廉与多通道的特点。结合自行研制的图像处理软件，对96孔微孔板样品的检测与数据处理可在10min内完成，实现了溶液的高通量定量检测。检测罗丹明B的灵敏度为（2．80±0．15）nmol／L。应用本装置检测水溶液中H矿，检出限为7．7μg/L；检测自来水样品中Hg^2＋的加标回收率为99．9％～102．4％，RSD为1．5％～2．6％；检测湖水样品中H矿的加标回收率为96．2％～98．9％，RSD为1．6％～2．5％。 |
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| AbstractList | 基于一字线激光模组,设计并构建了高通量便携式荧光检测装置,因其无需传统微孔板荧光仪所要求的专业精密调节的复杂光路系统,具有结构简单、小巧轻便、成本低廉与多通道的特点.结合自行研制的图像处理软件,对96孔微孔板样品的检测与数据处理可在10 min内完成,实现了溶液的高通量定量检测.检测罗丹明B的灵敏度为(2.80±0.15) nmol/L.应用本装置检测水溶液中Hg2+,检出限为7.7μg/L；检测自来水样品中Hg2+的加标回收率为99.9％～ 102.4％,RSD为1.5％～2.6％；检测湖水样品中Hg2+的加标回收率为96.2％～ 98.9％,RSD为1.6％～2.5％. 基于一字线激光模组，设计并构建了高通量便携式荧光检测装置，因其无需传统微孔板荧光仪所要求的专业精密调节的复杂光路系统，具有结构简单、小巧轻便、成本低廉与多通道的特点。结合自行研制的图像处理软件，对96孔微孔板样品的检测与数据处理可在10min内完成，实现了溶液的高通量定量检测。检测罗丹明B的灵敏度为（2．80±0．15）nmol／L。应用本装置检测水溶液中H矿，检出限为7．7μg/L；检测自来水样品中Hg^2＋的加标回收率为99．9％～102．4％，RSD为1．5％～2．6％；检测湖水样品中H矿的加标回收率为96．2％～98．9％，RSD为1．6％～2．5％。 |
| Author | 刘佳佳 郭祥群 |
| AuthorAffiliation | 厦门大学化学化工学院化学系,厦门361005 |
| AuthorAffiliation_xml | – name: 厦门大学化学化工学院化学系,厦门,361005 |
| Author_FL | LIU Jia-Jia GUO Xiang-Qun |
| Author_FL_xml | – sequence: 1 fullname: LIU Jia-Jia – sequence: 2 fullname: GUO Xiang-Qun |
| Author_xml | – sequence: 1 fullname: 刘佳佳 郭祥群 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3724/SP.J.1096.2013.30013 |
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| DocumentTitleAlternate | Development and Application of High-throughput and Portable Fluorescence Device |
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| EndPage | 1624 |
| ExternalDocumentID | fxhx201310028 47427974 |
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| Keywords | High-throughput Portable Line laser pointer 便携式 一字线激光模组 Fluorescence device 高通量 荧光检测装置 |
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| Notes | High-throughput ; Portable ; Fluorescence device ; Line laser pointer LIU Jia-Jia, GUO Xiang-Qun ＊ （Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China） 22-1125/O6 Muhichannel and high-throughput fluorescence measuring systems provide a great advantage in speeding up the batch analysis from several-fold to hundred-fold, and would be practically significant in application areas such as hygiene analysis, environmental analysis, food analysis, pharmaceutical analysis, biochemical and clinical analysis. Herein we report the design and fabrication of a novel high-throughput and portable fluorescence device. A line laser pointer was used as the light source, so as to avoid the drawbacks and limitations of conventional complicated optical setup which need professional and exact adjustment. The as-constructed device is simple, portable, low-cost and multichannel. It takes less than 10 min to collect and process data for a 96 microplate-batch analysis of solutio |
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| PublicationTitle | 分析化学 |
| PublicationTitleAlternate | Chinese Journal of Analytical Chemistry |
| PublicationTitle_FL | Chinese Journal of Analytical Chemistry |
| PublicationYear | 2013 |
| Publisher | 厦门大学化学化工学院化学系,厦门,361005 |
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| SubjectTerms | 一字线激光模组 便携式 荧光检测装置 高通量 |
| Title | 高通量便携式荧光检测装置的研制与应用 |
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