高通量便携式荧光检测装置的研制与应用
基于一字线激光模组，设计并构建了高通量便携式荧光检测装置，因其无需传统微孔板荧光仪所要求的专业精密调节的复杂光路系统，具有结构简单、小巧轻便、成本低廉与多通道的特点。结合自行研制的图像处理软件，对96孔微孔板样品的检测与数据处理可在10min内完成，实现了溶液的高通量定量检测。检测罗丹明B的灵敏度为（2．80±0．15）nmol／L。应用本装置检测水溶液中H矿，检出限为7．7μg/L；检测自来水样品中Hg^2＋的加标回收率为99．9％～102．4％，RSD为1．5％～2．6％；检测湖水样品中H矿的加标回收率为96．2％～98．9％，RSD为1．6％～2．5％。...
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| Published in | 分析化学 Vol. 41; no. 10; pp. 1620 - 1624 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
厦门大学化学化工学院化学系,厦门,361005
2013
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| Subjects | |
| Online Access | Get full text |
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| Summary: | 基于一字线激光模组，设计并构建了高通量便携式荧光检测装置，因其无需传统微孔板荧光仪所要求的专业精密调节的复杂光路系统，具有结构简单、小巧轻便、成本低廉与多通道的特点。结合自行研制的图像处理软件，对96孔微孔板样品的检测与数据处理可在10min内完成，实现了溶液的高通量定量检测。检测罗丹明B的灵敏度为（2．80±0．15）nmol／L。应用本装置检测水溶液中H矿，检出限为7．7μg/L；检测自来水样品中Hg^2＋的加标回收率为99．9％～102．4％，RSD为1．5％～2．6％；检测湖水样品中H矿的加标回收率为96．2％～98．9％，RSD为1．6％～2．5％。 |
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| Bibliography: | High-throughput ; Portable ; Fluorescence device ; Line laser pointer LIU Jia-Jia, GUO Xiang-Qun ＊ （Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China） 22-1125/O6 Muhichannel and high-throughput fluorescence measuring systems provide a great advantage in speeding up the batch analysis from several-fold to hundred-fold, and would be practically significant in application areas such as hygiene analysis, environmental analysis, food analysis, pharmaceutical analysis, biochemical and clinical analysis. Herein we report the design and fabrication of a novel high-throughput and portable fluorescence device. A line laser pointer was used as the light source, so as to avoid the drawbacks and limitations of conventional complicated optical setup which need professional and exact adjustment. The as-constructed device is simple, portable, low-cost and multichannel. It takes less than 10 min to collect and process data for a 96 microplate-batch analysis of solutio |
| ISSN: | 0253-3820 |
| DOI: | 10.3724/SP.J.1096.2013.30013 |