Effects of long-term exposure to 50 Hz magnetic fields on cell viability, genetic damage, and sensitivity to mutagen-induced damage

• Published in: Heliyon Vol. 9; no. 3; p. e14097
• Main Authors: Nguyen, Ha, Segers, Seppe, Ledent, Maryse, Anthonissen, Roel, Verschaeve, Luc, Hinsenkamp, Maurice, Collard, Jean-Francois, Feipel, Veronique, Mertens, Birgit
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2023; Elsevier
• Subjects: Adaptive response; ATP-based assay; cell lines; cell viability; chronic exposure; Co-exposure; comet assay; cytokinesis; Electromagnetic fields; Genotoxicity; human health; humans; magnetism; micronucleus tests; Non-ionizing radiation; viability assays; ATP-based assay; Non-ionizing radiation; Electromagnetic fields; Co-exposure; Genotoxicity; Adaptive response
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2023.e14097

Until today, it remains controversial whether long-term exposure to extremely low-frequency magnetic fields (ELF-MF) below the legislative exposure limits could result in adverse human health effects. In the present study, the effects of long-term in vitro MF exposure on three different study endpoints (cell viability, genetic damage, and sensitivity to damage induced by known mutagens) were investigated in the human B lymphoblastoid (TK6) cell line. Cells were exposed to 50 Hz MF at three selected magnetic flux densities (i.e., 10, 100, and 500 μT) for different exposure periods ranging from 96h up to 6 weeks. Cell viability following MF exposure was assessed using the ATP-based cell viability assay. Effects of MF exposure on cell genetic damage and cell sensitivity to mutagen-induced damage were evaluated using the in vitro alkaline comet assay and the in vitro cytokinesis block micronucleus assay. The results showed that long-term exposure up to 96h to 50 Hz MF at all tested flux densities could significantly increase TK6 cell viability. In contrast, long-term MF exposure did not affect cell genetic damage, and long-term pre-exposure to MF did not change cell sensitivity to damage induced by known mutagens. At certain time points, statistically significant difference in genotoxicity test results were observed between the MF-exposed cells and the control cells. However, these observations could not be confirmed in the repeat experiments, indicating that they are probably not biologically significant. •Optimized experiments to study the effects of long-term exposure to magnetic field.•Continuous exposure to 50 Hz magnetic field significantly increased TK6 viability.•Long-term exposure to 50 Hz magnetic field did not affect cell genetic damage.•Long-term pre-exposure to 50 Hz magnetic field did not induce an adaptive response.