Occurrence of herpes simplex virus 1 and three periodontal bacteria in patients with chronic periodontitis and necrotic pulp

• Published in: Canadian journal of microbiology Vol. 54; no. 4; pp. 326 - 330
• Main Authors: Nishiyama, Sheila Alexandra Belini, Nakano, Viviane, Velásquez-Melendez, Gustavo, Avila-Campos, Mario Julio
• Format: Journal Article
• Language: English
• Published: Ottawa, ON National Research Council of Canada 01.04.2008; NRC Research Press; Canadian Science Publishing NRC Research Press
• Subjects: Adolescent; Adult; Bacteria; Bacteriology; Bacteroides forsythus; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Biological and medical sciences; Causes of; Chronic Disease; Chronic illnesses; chronic periodontitis; Dental Pulp - microbiology; Dental Pulp - pathology; Dental Pulp - virology; Diagnosis; Dialister pneumosintes; Epidemiology; Female; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - genetics; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacterial Infections - epidemiology; Gram-Negative Bacterial Infections - microbiology; Health aspects; herpes simples virus; Herpes Simplex - epidemiology; Herpes Simplex - virology; Herpes simplex virus; Herpes simplex virus 1; Herpes viruses; Herpesvirus 1, Human - genetics; Herpesvirus 1, Human - isolation & purification; Humans; Male; Microbiology; Middle Aged; Necrosis - epidemiology; Necrosis - microbiology; Necrosis - virology; necrotic pulp; Periodontal disease; Periodontitis - epidemiology; Periodontitis - microbiology; Periodontitis - virology; Polymerase Chain Reaction; pulpe nécrotique; périodontite chronique; Studies; Tannerella forsythia; Treponema; Treponema denticola; Treponema denticola - genetics; Treponema denticola - isolation & purification; Veillonellaceae - genetics; Veillonellaceae - isolation & purification; Virology; virus herpes simplex; Brazil; Human; Treponema denticola; Spirochaetales; Stomatology; Herpesviridae; Alphaherpesvirinae; Herpes; Bacteroidaceae; Infection; Virus; Chronic; Periodontal disease; Periodontitis; Viral disease; Tannerella forsythensis; Bacteria; Human herpesvirus 1; Treponemataceae
• ISSN: 0008-4166; 1480-3275
• DOI: 10.1139/W08-006

Viral and bacterial associations appear to be implicated in the development of periodontal infections. Little information is available describing the periodontopathic agents in root canals with necrotic pulp. In this study, the occurrence and the combinations among herpes simplex virus type 1 (HSV-1) and Dialister pneumosintes , Tannerella forsythia , and Treponema denticola in patients with chronic periodontitis and necrotic pulp were evaluated. Clinical samples from healthy subjects and patients with periodontal or pulp infections were analyzed using a nested polymerase chain reaction PCR to detect HSV and PCR to detect the 3 periodontal bacteria. The presence of Tannerella forsythia and Treponema denticola was observed in healthy, periodontitis, and necrotic pulp patients. HSV was observed in periodontitis and necrotic pulp patients, and no healthy subject harbored D. pneumosintes or HSV. The occurrence of Tannerella forsythia was not statistically significant in patients with necrotic pulp (P = 0.704). Periodontal bacteria were observed varying from 10.3% to 20.7% in periodontitis and necrotic pulp patients. The presence of Treponema denticola - HSV association was predominant in patients showing necrotic pulp (24.1%); however, HSV alone was observed in one patient with periodontitis and in another patient with necrotic pulp. The presence of double association among bacteria or bacteria - HSV could indicate a role in both periodontitis and necrotic pulp, and Tannerella forsythia - Treponema denticola - HSV and Tannerella forsythia - D. pneumosintes - Treponema denticola - HSV associations might be important in periodontitis.