Characterization of C-phycocyanin antioxidant, anti-inflammatory, anti-tumour, and anti-HCoV-229E activities and encapsulation for implementation in an innovative functional yogurt

• Published in: Heliyon Vol. 10; no. 11; p. e31642
• Main Authors: Soliman, Tarek Nour, Negm El-Dein, Asmaa, Abd Al-Daim, Sahar, Allayeh, Abdou, Awad, Hanem, Flefil, Nasser S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.06.2024; Elsevier
• Subjects: adsorption; amino acid composition; antioxidants; C-Phycocyanin; colon; cytotoxicity; doxorubicin; encapsulation; Functional yogurt; Human coronavirus 229E; humans; inflammation; Lactobacillus rhamnosus; liver; microalgae; milk; molecular weight; Nano-phytosome; nanoparticles; particle size; Pharmacological properties; pharmacology; phycocyanin; probiotics; Spirulina platensis; yogurt; zeta potential; C-Phycocyanin; Functional yogurt; Human coronavirus 229E; Nano-phytosome; Spirulina platensis; Pharmacological properties
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e31642

Functional bioactive ingredients isolated from microalgae as sustainable sources have become a new subject of pharmacology and functional foods. Thus, the work aims to produce crude phycocyanin (C-PC), define it, and investigate its pharmacological effects before warping it in a nanophytosome. Subsequently, the physicochemical properties of nanoparticles were evaluated. Both free and nanophytosomes of C-PC were incorporated into cow milk fermented with the probiotic Lactobacillus rhamnosus KU985435 to make functional yoghurt and the stability of C-PC of both phytosomes was assessed. The amino acid content of C-PC revealed the presence of eight of nine essential amino acids and eight of eleven non-essential amino acids. C-PC has a medium molecular weight (82.992 kDa). Some pharmacological effects like reducing inflammation (98.76 % ± 0.065), fighting free radicals (99.12 % ± 0.027), and being able to inhibit the human coronavirus 229 E with a selective index of 27.9 were observed. The maximum viral inhibitory activity was detected during the adsorption stage. Anti-human liver and colon carcinomas that exceeded Doxorubicin with very low cytotoxicity against normal cell lines were detected. C-PC is an unstable protein that could be degraded in the yoghurt during storage. Therefore, phytosome encapsulation can effectively stabilize C-PC (particle size 44.50 ± 12 nm and zeta-potential −32.4 ± 5 mV) and protect it from the acidic environment of the yoghurt. The produced yoghurt showed the desired physicochemical and functional properties and overall acceptance. The results prove that C-PC from spirulina algae is a renewable source of dyes. The encapsulation process using phytosomes gave it high stability against environmental influences, and therefore, it can be applied in the food and pharmaceutical industries in the future.