Direct Determination of Zinc in Human Serum by Flow-Injection Spectrophotometric Analysis
Flow-injection spectrophotometric analysis has been established for the determination of zinc at 1μg ml-1 level in serum using 5-Br-PAPS(2-(5-bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol, disodium salt) as a chromogenic reagent. A 100μl portion of serum is directly injected into t...
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Published in | Analytical Sciences Vol. 10; no. 5; pp. 795 - 799 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Tokyo
The Japan Society for Analytical Chemistry
1994
Japan Society for Analytical Chemistry Japan Science and Technology Agency |
Subjects | |
Online Access | Get full text |
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Summary: | Flow-injection spectrophotometric analysis has been established for the determination of zinc at 1μg ml-1 level in serum using 5-Br-PAPS(2-(5-bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol, disodium salt) as a chromogenic reagent. A 100μl portion of serum is directly injected into the carrier stream containing 5%(v/v) TMAH (tetramethylammonium hydroxide) and 0.5M trisodium citrate. After mixing with 0.01%(w/v) 5-Br-PAPS-0.5M ammonium acetate-7.2×10-3M salicylaldehyde oxime-1%(v/v) Triton X-100-0.5M hydrochloric acid solution, the absorbance of Zn-5-Br-PAPS complex is measured at 560nm. The proposed flow-injection system is fully controlled with a personal computer and permits 60 samples to be measured per hour. The relative standard deviation (n=50) for 1.2μgZn ml-1 solution is 3.3%. |
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ISSN: | 0910-6340 1348-2246 |
DOI: | 10.2116/analsci.10.795 |