Peptostreptococcus faecalis sp. nov., new bacterial species isolated from healthy indigenous congolese volunteer

• Published in: Heliyon Vol. 8; no. 3; p. e09102
• Main Authors: Mekhalif, Fatima, Zgheib, Rita, Akiana, Jean, Bilen, Melhem, Ndombe, Geor Mongo, Fenollar, Florence, Fournier, Pierre-Edouard, Raoult, Didier, Alibar, Stéphane, Mediannikov, Oleg, Lo, Cheikh Ibrahima
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2022; Elsevier
• Subjects: Bacteriology; Cardiology and cardiovascular system; Culturomics; Emerging diseases; Gut microbiote; Human health and pathology; Indigenous congolese; Infectious diseases; Life Sciences; Microbiology and Parasitology; Parasitology; Peptostreptococcus faecalis; Taxonogenomics; Virology; Taxonogenomics; Indigenous congolese; Gut microbiote; Peptostreptococcus faecalis; Culturomics
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2022.e09102

The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308T from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308T is a Gram-positive coccus shaped bacterium that optimally grows at 37 °C. The 16S rRNA gene sequence of the strain has a 96.2% sequence similarity to Peptostreptococcus anaerobius strain NCTC 11460T (GenBank accession number: NR_042847.1). In addition, the average nucleotide identity of strain Marseille-P4308T with its closest related species was 71.1%, which was far below the recommended threshold (>95–96%). The genome of the strain Marseille-P4308T has a length of 2.14 Mbp with G + C content of 30.4 mol%. Based on phenotypic, biochemical, genomic and phylogenetic analysis, strain Marseille-P4308T (= CSUR P4308 = CECT 9960) clearly appears to be a new species for which the name Peptostreptococcus faecalis sp. nov., is proposed. Peptostreptococcus faecalis; Gut microbiote; Indigenous congolese; Culturomics; Taxonogenomics