Fast wide-field upconversion luminescence lifetime thermometry enabled by single-shot compressed ultrahigh-speed imaging

• Published in: Nature communications Vol. 12; no. 1; p. 6401
• Main Authors: Liu, Xianglei, Skripka, Artiom, Lai, Yingming, Jiang, Cheng, Liu, Jingdan, Vetrone, Fiorenzo, Liang, Jinyang
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.11.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 639/301/930/2735; 639/624/1107/328; 639/624/1107/510; 639/624/399/354; Algorithms; Erbium; Humanities and Social Sciences; Imaging; Indicators; Luminescence; Mapping; Monitoring instruments; multidisciplinary; Nanoparticles; New records; Optical instruments; Photoluminescence; Photons; Science; Science & Technology - Other Topics; Science (multidisciplinary); Thermometry; Upconversion; Video compression; Ytterbium
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-26701-1

Photoluminescence lifetime imaging of upconverting nanoparticles is increasingly featured in recent progress in optical thermometry. Despite remarkable advances in photoluminescent temperature indicators, existing optical instruments lack the ability of wide-field photoluminescence lifetime imaging in real time, thus falling short in dynamic temperature mapping. Here, we report video-rate upconversion temperature sensing in wide field using single-shot photoluminescence lifetime imaging thermometry (SPLIT). Developed from a compressed-sensing ultrahigh-speed imaging paradigm, SPLIT first records wide-field luminescence intensity decay compressively in two views in a single exposure. Then, an algorithm, built upon the plug-and-play alternating direction method of multipliers, is used to reconstruct the video, from which the extracted lifetime distribution is converted to a temperature map. Using the core/shell NaGdF 4 :Er 3+ ,Yb 3+ /NaGdF 4 upconverting nanoparticles as the lifetime-based temperature indicators, we apply SPLIT in longitudinal wide-field temperature monitoring beneath a thin scattering medium. SPLIT also enables video-rate temperature mapping of a moving biological sample at single-cell resolution. Photoluminescence lifetime imaging of upconverting nanoparticles is useful for optical thermometry, but is limited for dynamic samples. Here, the authors present a wide-field and single shot approach based on compressive sensing, for video-rate upconversion temperature sensing of moving samples.