Subinhibitory concentrations of fluconazole increase the intracellular sodium content in both fluconazole-resistant and -sensitive Candida albicans strains

• Published in: Canadian journal of microbiology Vol. 55; no. 5; pp. 605 - 610
• Main Authors: Kolecka, Anna, Krauke, Yannick, Bujdakova, Helena, Sychrova, Hana
• Format: Journal Article
• Language: English
• Published: Ottawa, ON National Research Council of Canada 01.05.2009; NRC Research Press; Canadian Science Publishing NRC Research Press
• Subjects: Antifungal Agents - pharmacology; Bacteria; Biological and medical sciences; Candida albicans; Candida albicans - chemistry; Candida albicans - drug effects; Candida albicans - growth & development; Candidiasis; Cations; Causes of; Comparative analysis; Culture Media - chemistry; Cytosol - chemistry; Dosage and administration; Drug resistance in microorganisms; Drug Resistance, Fungal; Drug therapy; Fluconazole; Fluconazole - pharmacology; fluconazole resistance; Fundamental and applied biological sciences. Psychology; Health aspects; Humans; Microbiology; Miscellaneous; Mycology; Osmotic Pressure; osmotolerance; osmotolérance; Physical growth; résistance au fluconazole; salt sensitivity; sensibilité aux sels; Sodium; Sodium - analysis; Sodium - pharmacology; Sodium chloride; Temperature; Czech Republic; Candida albicans; Yeast; Fluconazole; Azole derivatives; Strain; Fungi; Resistance; Antifungal agent; Sensitivity; Sensitivity resistance; Sodium; Triazole derivatives; Fungi Imperfecti; Intracellular
• ISSN: 0008-4166; 1480-3275
• DOI: 10.1139/W09-009

Fluconazole-sensitive (SC 5314) and -resistant (clinical isolate 1173) Candida albicans strains were compared in terms of their osmotolerance and tolerance to toxic sodium cations. The two strains did not differ in their tolerance to high osmotic pressure in general but exhibited distinct sensitivities to sodium cations. Although the fluconazole-resistant 1173 strain contained, under all conditions tested, significantly lower intracellular amounts of Na + , it was much more sodium sensitive than the SC 5314 strain. The addition of subinhibitory concentrations of fluconazole to media supplemented with NaCl significantly influenced the growth of both strains and resulted in substantially elevated intracellular sodium concentrations compared with growth in medium containing NaCl but no fluconazole.