Hyaluronan is apically secreted and expressed by proliferating or regenerating renal tubular cells

• Published in: Kidney international Vol. 68; no. 1; pp. 71 - 83
• Main Authors: Asselman, Marino, Verhulst, Anja, Van Ballegooijen, Eddy S., Bangma, Chris H., Verkoelen, Carl F., De Broe, Marc E.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.07.2005; Nature Publishing; Elsevier Limited
• Subjects: Animals; Antimetabolites; Base Sequence; Biological and medical sciences; Bromodeoxyuridine; CD44; Cell Division - physiology; Cell Line; Cell Polarity - physiology; Dogs; Gene Expression Regulation, Enzymologic; Glucuronosyltransferase - genetics; Humans; hyaluronan; Hyaluronan Receptors - metabolism; Hyaluronan Synthases; Hyaluronic Acid - biosynthesis; Hyaluronic Acid - metabolism; Hyaluronic Acid - secretion; Kidney Tubular Necrosis, Acute - metabolism; Kidney Tubular Necrosis, Acute - physiopathology; Kidney Tubules - cytology; Kidney Tubules - metabolism; Medical sciences; Microscopy, Confocal; Molecular Sequence Data; Nephrology. Urinary tract diseases; polarity; Regeneration - physiology; renal stone disease; renal tubular cells; Reverse Transcriptase Polymerase Chain Reaction; Tritium; renal tubular cells; hyaluronan synthases; renal stone disease; hyaluronan; CD44; polarity; Nephrology; Enzyme; Secretion; Synthase; Kidney; Urology; Renal tubule; Urinary system; Healing agent; Lithiasis; Glycosaminoglycan; Polarity; Antirheumatic agent; Hyaluronic acid; Cell
• ISSN: 0085-2538; 1523-1755
• DOI: 10.1111/j.1523-1755.2005.00382.x

Hyaluronan is apically secreted and expressed by proliferating or regenerating renal tubular cells. Hyaluronan has diverse biologic functions in the body, varying from structural tasks to cell stress-induced CD44-mediated activation of intracellular signaling pathways. Hyaluronan biology is relatively unexplored in the kidney. Previously, we identified hyaluronan as binding molecule for crystals in the renal tubules. Crystal retention is a crucial early event in the etiology of kidney stones. The present study was performed to determine the polarized distribution of hyaluronan and CD44 by renal tubular cells. Madin-Darby canine kidney (MDCK) strain I and primary cultures of human renal tubular cells were grown on permeable supports in a two-compartment culture system. Studies were performed during growth and after scrape-injury. Metabolic labeling studies and an enzyme-linked hyaluronan -binding assay were used to measure the molecular mass and the amount of secreted hyaluronan in apical and basal medium. Confocal microscopy was applied to detect membrane hyaluronan and CD44. Hyaluronan synthase (HAS) mRNA expression was studied with reverse transcriptase-polymerase chain reaction (RT-PCR). The in vitro expression profile of hyaluronan was compared with that in biopsies of transplanted human kidneys with acute tubular necrosis. Proliferating cells produced more hyaluronan (Mr > 106 Da) than growth-inhibited cells in intact monolayers and up to 85% was targeted to the apical compartment, which was accompanied by increased HAS2 mRNA expression and slightly decreased HAS3 mRNA, while HAS1 mRNA remained undetectable. Hyaluronan and CD44 were exclusively expressed at the apical surface of proliferating/regenerating cells. After (re)establishment of tight junctions, hyaluronan was no longer detectable while CD44 was targeted to basolateral membrane domains. In vivo in inflamed human kidneys hyaluronan was abundantly expressed in the cortical tubulointerstitial space as well as at the luminal surface of regenerating renal tubular cells. These results demonstrate that the production of hyaluronan by renal tubular cells is activated during proliferation and in response to mechanical injury and that hyaluronan and CD44 expression is highly polarized. The targeted delivery of hyaluronan to the apical compartment suggests that hyaluronan produced by renal tubular cells supports proliferation/regeneration in the renal tubules, but that it does not contribute to hyaluronan accumulation in the renal interstitium. These data further support the concept that mitogen/stress-induced hyaluronan deposition in the renal tubules increases the risk for crystal retention and stone formation.