Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays

Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a "chemical nose/tongue" approach that exploits subtle cha...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 106; no. 27; pp. 10912 - 10916
Main Authors Bajaj, Avinash, Miranda, Oscar R, Kim, Ik-Bum, Phillips, Ronnie L, Jerry, D. Joseph, Bunz, Uwe H.F, Rotello, Vincent M
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 07.07.2009
National Acad Sciences
Subjects
Animals
Biological Sciences
Biosensing Techniques - instrumentation
Cell division
Cell Line, Tumor
Cells
Humans
Metastasis
Mice
Nanoparticles
Nanoparticles - chemistry
Neoplasm Metastasis - diagnosis
Neoplasm Metastasis - pathology
Neoplasms - diagnosis
Neoplasms - pathology
Physical Sciences
Polymers
Polymers - chemistry
Sensors
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Summary:Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a "chemical nose/tongue" approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.
Bibliography:Edited by Laura L. Kiessling, University of Wisconsin, Madison, WI, and approved May 20, 2009
Author contributions: A.B., D.J.J., U.H.F.B., and V.M.R. designed research; A.B. and O.R.M. performed research; I.-B.K. and R.L.P. contributed new reagents/analytic tools; A.B., O.R.M., and V.R. analyzed data; and A.B., O.R.M., D.J.J., U.H.F.B., and V.M.R. wrote the paper.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0900975106