An Atlas of Vagal Sensory Neurons and Their Molecular Specialization

• Published in: Cell reports (Cambridge) Vol. 27; no. 8; pp. 2508 - 2523.e4
• Main Authors: Kupari, Jussi, Häring, Martin, Agirre, Eneritz, Castelo-Branco, Gonçalo, Ernfors, Patrik
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.05.2019; Cell Press; Elsevier
• Subjects: Animals; Female; jugular ganglion; Male; mechanoreceptor; Mice; Mice, Inbred C57BL; Mice, Transgenic; Neurons - cytology; Neurons - metabolism; nociceptor; nodose ganglion; Nodose Ganglion - cytology; Nodose Ganglion - metabolism; sensory neurons; Sequence Analysis, RNA; single cell RNA-sequencing; Single-Cell Analysis; somatosensory; Transcriptome; vagus nerve; Vagus Nerve - cytology; Vagus Nerve - metabolism; viscerosensory; vagus nerve; somatosensory; nociceptor; nodose ganglion; jugular ganglion; mechanoreceptor; viscerosensory; transcriptome; single cell RNA-sequencing; sensory neurons
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2019.04.096

Sensory functions of the vagus nerve are critical for conscious perceptions and for monitoring visceral functions in the cardio-pulmonary and gastrointestinal systems. Here, we present a comprehensive identification, classification, and validation of the neuron types in the neural crest (jugular) and placode (nodose) derived vagal ganglia by single-cell RNA sequencing (scRNA-seq) transcriptomic analysis. Our results reveal major differences between neurons derived from different embryonic origins. Jugular neurons exhibit fundamental similarities to the somatosensory spinal neurons, including major types, such as C-low threshold mechanoreceptors (C-LTMRs), A-LTMRs, Aδ-nociceptors, and cold-, and mechano-heat C-nociceptors. In contrast, the nodose ganglion contains 18 distinct types dedicated to surveying the physiological state of the internal body. Our results reveal a vast diversity of vagal neuron types, including many previously unanticipated types, as well as proposed types that are consistent with chemoreceptors, nutrient detectors, baroreceptors, and stretch and volume mechanoreceptors of the respiratory, gastrointestinal, and cardiovascular systems. [Display omitted] •A comprehensive molecular identification of neuronal types in vagal ganglion complex•Prdm12+ jugular ganglion neurons share features with spinal somatosensory neurons•Phox2b+ viscerosensory nodose neurons are molecularly versatile and highly specialized•Nodose neuron types are consistent with chemo-, baro-, stretch-, tension-, and volume-sensors Visceral sensory neurons are necessary for the control of organ functions, but knowledge on the complexity of neuron types involved is missing. Kupari et al. molecularly identify jugular and nodose ganglion neurons and find a large diversity of neuron types that are consistent with the numerous sensory functions of the vagus nerve.