Reassessing the genetic variability of Tectona grandis through high-throughput genotyping: Insights on its narrow genetic base

• Published in: PloS one Vol. 18; no. 10; p. e0285518
• Main Authors: dos Anjos, Isabela Vera, Gilio, Thiago Alexandre Santana, Amorim, Ana Flávia S, de Jesus, Jeferson Gonçalves, Chimello, Antonio Marcos, Takizawa, Fausto H, Araujo, Kelly Lana, Neves, Leonarda Grillo
• Format: Journal Article
• Language: English
• Published: San Francisco, CA USA Public Library of Science 26.10.2023; Public Library of Science (PLoS)
• Subjects: Analysis; Biology and Life Sciences; Computer and Information Sciences; DNA sequencing; Ecology and Environmental Sciences; Genetic aspects; Genomes; Nucleotide sequencing; People and Places; Teak; Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0285518

Teak (Tectona grandis Linn. f.) is considered one of the most expensive hardwoods in the world. The dispersion of the species over the years has taken the teak beyond its first sources of diversity and little is known about the genetic origin and genetic variability. Thus, this study aimed to investigate the genetic diversity and genetic population structure existing in a representative teak germplasm bank collection. DNA was extracted from young leaves and each sample were genotyped by whole genome sequencing at 3 giga bases per sample, the sequences are aligned using the genome, and SNPcalls and quality control were made. To study the population structure of the genotypes, Bayesian variational inference was used via fastStructure, the phylogenetic tree was based on the modified Euclidean distance and the clustering by the UPGMA hierarchical method. Genetic diversity was analyzed based on the pairwise genetic divergence (Fst) of Weir and Cockerham. Genotyping by sequencing resulted in a database of approximately 1.4 million of variations SNPs were used for analysis. It was possible to identify four populations with considerable genetic variability between and within them. While the genetic variability in teak is generally known to be narrow, this study confirmed the presence of genetic variability scale in teak, which is contrary to what was initially expected.