Evaluation of Chemical Analysis Method and Determination of Polycyclic Aromatic Hydrocarbons Content from Seafood and Dairy Products

• Published in: Toxicological research (Seoul) Vol. 31; no. 3; pp. 265 - 271
• Main Authors: Lee, So-Young, Lee, Jee-Yeon, Shin, Han-Seung
• Format: Journal Article
• Language: English
• Published: Singapore 한국독성학회 01.09.2015; Springer Singapore; The Korean Society Of Toxicology
• Subjects: Biomedicine; Original Article; Pharmacology/Toxicology; 예방의학; Seafood; Carcinogen; Dairy products; Polycyclic aromatic hydrocarbon
• ISSN: 1976-8257; 2234-2753
• DOI: 10.5487/TR.2015.31.3.265

This study was carried out to investigate contents of 8 polycyclic aromatic hydrocarbons (PAHs) from frequently consumed seafood and dairy products and to evaluate their chemical analysis methods. Samples were collected from markets of 9 cities in Korea chosen as the population reference and evaluated. The methodology involved saponification, extraction with n-hexane, clean-up on Sep-Pak silica cartridges and gas chromatograph-mass spectrometry analysis. Validation proceeded on 2 matrices. Recoveries for 8 PAHs ranged from 86.87 to 103.57%. The limit of detection (LOD) 8 PAHs was 0.04~0.20 µg/kg, and limit of quantification (LOQ) of 8 PAHs was 0.12~0.60 µg/kg. The mean concentration of benzo[a]pyrene (BaP) was 0.34 µg/kg from seafood and 0.34 µg/kg from dairy products. The total PAHs concentration was 1.06 µg/kg in seafood and 1.52 µg/kg in dairy products.