Virus-mimetic nanovesicles as a versatile antigen-delivery system
It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission,...
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| Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 45; pp. E6129 - E6138 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
National Academy of Sciences
10.11.2015
National Acad Sciences |
| Series | PNAS Plus |
| Subjects | |
| Online Access | Get full text |
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| Abstract | It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission, we genetically engineered viral antigen to harbor into cell membrane, then form uniform spherical virus-mimetic nanovesicles (VMVs) that resemble natural virus in size, shape, and specific immunogenicity with the help of surfactants. Incubation of major cell membrane vesicles with surfactants generates a large amount of nano-sized uniform VMVs displaying the native conformational epitopes. With the diverse display of epitopes and viral envelope glycoproteins that can be functionally anchored onto VMVs, we demonstrate VMVs to be straightforward, robust and tunable nanobiotechnology platforms for fabricating antigen delivery systems against a wide range of enveloped viruses. |
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| AbstractList | The previously unidentified virus-mimetic nanovesicles (VMVs) described in this manuscript consist of phospholipid derived from mammalian cell plasma membrane, recombinant protein anchored to cell membrane via the route of signal peptide sorting, and surfactants capable of controlling the VMV size and strength, which allows the VMVs to display functional polypeptides or maintain the correct conformation of protein antigen. The protein integrated into VMV by its hydrophobic transmembrane peptide has more modifications, such as glycosylation, than proteins in conventional subunit vaccines. Moreover, many viral envelope glycoproteins can be genetically engineered onto VMV liposomal surface so as to mimic the properties and conformational epitopes of natural virus. VMV provides an effective, straightforward, and tunable approach against a wide range of emerging enveloped viruses.
It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission, we genetically engineered viral antigen to harbor into cell membrane, then form uniform spherical virus-mimetic nanovesicles (VMVs) that resemble natural virus in size, shape, and specific immunogenicity with the help of surfactants. Incubation of major cell membrane vesicles with surfactants generates a large amount of nano-sized uniform VMVs displaying the native conformational epitopes. With the diverse display of epitopes and viral envelope glycoproteins that can be functionally anchored onto VMVs, we demonstrate VMVs to be straightforward, robust and tunable nanobiotechnology platforms for fabricating antigen delivery systems against a wide range of enveloped viruses. It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission, we genetically engineered viral antigen to harbor into cell membrane, then form uniform spherical virus-mimetic nanovesicles (VMVs) that resemble natural virus in size, shape, and specific immunogenicity with the help of surfactants. Incubation of major cell membrane vesicles with surfactants generates a large amount of nano-sized uniform VMVs displaying the native conformational epitopes. With the diverse display of epitopes and viral envelope glycoproteins that can be functionally anchored onto VMVs, we demonstrate VMVs to be straightforward, robust and tunable nanobiotechnology platforms for fabricating antigen delivery systems against a wide range of enveloped viruses. |
| Author | Liu, Gang Wang, Zhantong Zheng, Qingbing Zeng, Yun Tian, Rui Zhang, Xianzhong Ge, Shengxiang Li, Shaowei Chu, Chengchao Zheng, Zizheng Chen, Xiaoyuan Xia, Ning-Shao Li, Rui Zhang, Pengfei Shen, Chenguang Chen, Yixin Guo, Zhide |
| Author_xml | – sequence: 1 givenname: Pengfei surname: Zhang fullname: Zhang, Pengfei organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 2 givenname: Yixin surname: Chen fullname: Chen, Yixin organization: State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, 361102, China – sequence: 3 givenname: Yun surname: Zeng fullname: Zeng, Yun organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 4 givenname: Chenguang surname: Shen fullname: Shen, Chenguang organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 5 givenname: Rui surname: Li fullname: Li, Rui organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 6 givenname: Zhide surname: Guo fullname: Guo, Zhide organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 7 givenname: Shaowei surname: Li fullname: Li, Shaowei organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 8 givenname: Qingbing surname: Zheng fullname: Zheng, Qingbing organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 9 givenname: Chengchao surname: Chu fullname: Chu, Chengchao organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 10 givenname: Zhantong surname: Wang fullname: Wang, Zhantong organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 11 givenname: Zizheng surname: Zheng fullname: Zheng, Zizheng organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 12 givenname: Rui surname: Tian fullname: Tian, Rui organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 13 givenname: Shengxiang surname: Ge fullname: Ge, Shengxiang organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 14 givenname: Xianzhong surname: Zhang fullname: Zhang, Xianzhong organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 15 givenname: Ning-Shao surname: Xia fullname: Xia, Ning-Shao organization: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102, China – sequence: 16 givenname: Gang surname: Liu fullname: Liu, Gang organization: The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China – sequence: 17 givenname: Xiaoyuan surname: Chen fullname: Chen, Xiaoyuan organization: Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892 |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26504197$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1021/nl500618u 10.1083/jcb.103.4.1179 10.1007/978-1-59745-483-4_31 10.1038/emboj.2012.87 10.1038/nnano.2012.212 10.1586/14760584.2015.1046440 10.1038/nrd3499 10.1083/jcb.121.1.171 10.3390/12102292 10.1016/j.immuni.2013.10.010 10.1111/j.1469-0691.2010.03413.x 10.1038/nri2567 10.1016/j.tibs.2006.08.004 10.1038/nnano.2007.223 10.1038/85438 10.1093/jnci/djp106 10.1111/j.1469-0691.2011.03538.x 10.1086/594374 10.1002/adma.201405634 10.1038/nnano.2013.254 10.1021/mp049964d 10.1038/nbt1261 10.1016/j.tibtech.2013.09.002 10.1021/la011421c 10.1083/jcb.111.2.409 10.1073/pnas.0805532107 10.1128/JVI.07164-11 10.1073/pnas.1409861111 10.1016/j.vaccine.2012.12.031 10.1128/JVI.00936-07 10.1038/nri3488 10.1038/ncomms5327 |
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| Copyright | Volumes 1–89 and 106–112, copyright as a collective work only; author(s) retains copyright to individual articles Copyright National Academy of Sciences Nov 10, 2015 |
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| Keywords | virus-mimetic vesicle vaccine nanobiotechnology antigen delivery system cell membrane |
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| Notes | SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23 1P.Z., Y.C., and Y.Z. contributed equally to this work. Author contributions: G.L. and X.C. designed research; P.Z., Y.C., Y.Z., C.S., R.L., Z.G., S.L., Q.Z., C.C., Z.W., Z.Z., R.T., and S.G. performed research; P.Z., Y.C., X.Z., N.-S.X., and G.L. analyzed data; and G.L. and X.C. wrote the paper. Edited by Omid C. Farokhzad, Brigham and Women's Hospital - Harvard Medical School, Boston, MA, and accepted by the Editorial Board September 30, 2015 (received for review March 23, 2015) |
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| Snippet | It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the... The previously unidentified virus-mimetic nanovesicles (VMVs) described in this manuscript consist of phospholipid derived from mammalian cell plasma membrane,... |
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| StartPage | E6129 |
| SubjectTerms | Antigens Antigens - metabolism Biological Sciences Drug Delivery Systems - methods Glycoproteins Immunogenicity Membranes Morbidity Mortality Nanostructures - chemistry Nanotechnology - methods Nanotechnology - trends Pandemics Phospholipids - analysis PNAS Plus Recombinant Proteins - metabolism Surface-Active Agents - analysis Surfactants Transport Vesicles - chemistry Vaccines Viral Vaccines - metabolism Viruses |
| Title | Virus-mimetic nanovesicles as a versatile antigen-delivery system |
| URI | https://www.jstor.org/stable/26466385 http://www.pnas.org/content/112/45/E6129.abstract https://www.ncbi.nlm.nih.gov/pubmed/26504197 https://www.proquest.com/docview/1734739291 https://www.proquest.com/docview/1762353136 https://pubmed.ncbi.nlm.nih.gov/PMC4653155 |
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