Virus-mimetic nanovesicles as a versatile antigen-delivery system

It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission,...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 112; no. 45; pp. E6129 - E6138
Main Authors Zhang, Pengfei, Chen, Yixin, Zeng, Yun, Shen, Chenguang, Li, Rui, Guo, Zhide, Li, Shaowei, Zheng, Qingbing, Chu, Chengchao, Wang, Zhantong, Zheng, Zizheng, Tian, Rui, Ge, Shengxiang, Zhang, Xianzhong, Xia, Ning-Shao, Liu, Gang, Chen, Xiaoyuan
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 10.11.2015
National Acad Sciences
SeriesPNAS Plus
Subjects
Antigens
Antigens - metabolism
Biological Sciences
Drug Delivery Systems - methods
Glycoproteins
Immunogenicity
Membranes
Morbidity
Mortality
Nanostructures - chemistry
Nanotechnology - methods
Nanotechnology - trends
Pandemics
Phospholipids - analysis
PNAS Plus
Recombinant Proteins - metabolism
Surface-Active Agents - analysis
Surfactants
Transport Vesicles - chemistry
Vaccines
Viral Vaccines - metabolism
Viruses
virus-mimetic vesicle
vaccine
nanobiotechnology
antigen delivery system
cell membrane
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Summary:It is a critically important challenge to rapidly design effective vaccines to reduce the morbidity and mortality of unexpected pandemics. Inspired from the way that most enveloped viruses hijack a host cell membrane and subsequently release by a budding process that requires cell membrane scission, we genetically engineered viral antigen to harbor into cell membrane, then form uniform spherical virus-mimetic nanovesicles (VMVs) that resemble natural virus in size, shape, and specific immunogenicity with the help of surfactants. Incubation of major cell membrane vesicles with surfactants generates a large amount of nano-sized uniform VMVs displaying the native conformational epitopes. With the diverse display of epitopes and viral envelope glycoproteins that can be functionally anchored onto VMVs, we demonstrate VMVs to be straightforward, robust and tunable nanobiotechnology platforms for fabricating antigen delivery systems against a wide range of enveloped viruses.
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1P.Z., Y.C., and Y.Z. contributed equally to this work.
Author contributions: G.L. and X.C. designed research; P.Z., Y.C., Y.Z., C.S., R.L., Z.G., S.L., Q.Z., C.C., Z.W., Z.Z., R.T., and S.G. performed research; P.Z., Y.C., X.Z., N.-S.X., and G.L. analyzed data; and G.L. and X.C. wrote the paper.
Edited by Omid C. Farokhzad, Brigham and Women's Hospital - Harvard Medical School, Boston, MA, and accepted by the Editorial Board September 30, 2015 (received for review March 23, 2015)
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1505799112