尺寸排阻色谱-电感耦合等离子体质谱联用技术研究牛血清白蛋白与顺铂的反应动力学
顺铂是广泛应用的抗肿瘤药物,生理盐水稀释后通过静脉注射到体内。研究顺铂和血浆蛋白间的相互作用对理解其药效十分重要。本实验利用尺寸排阻色谱-电感耦合等离子体质谱（SEC/ICP-MS）联用技术研究了顺铂和牛血清白蛋白（BSA）的反应速率常数。顺铂与BSA于37℃孵育3,6,9,17和24 h后,用SEC/ICP-MS分析顺铂及顺铂-BSA复合物铂,从而计算出顺铂和BSA的反应速率常数为0.077 h-1。方法对于铂的检出限为0.19 ng/g。...
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| Published in | 分析化学 Vol. 40; no. 8; pp. 1289 - 1292 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国科学院高能物理研究所核分析技术重点实验室和纳米生物效应实验室,北京100049%中国科学院高能物理研究所核分析技术重点实验室和纳米生物效应实验室,北京,100049%河北工业大学材料科学与工程学院,天津,300130
2012
河北工业大学材料科学与工程学院,天津300130 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2012.11344 |
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| Summary: | 顺铂是广泛应用的抗肿瘤药物,生理盐水稀释后通过静脉注射到体内。研究顺铂和血浆蛋白间的相互作用对理解其药效十分重要。本实验利用尺寸排阻色谱-电感耦合等离子体质谱（SEC/ICP-MS）联用技术研究了顺铂和牛血清白蛋白（BSA）的反应速率常数。顺铂与BSA于37℃孵育3,6,9,17和24 h后,用SEC/ICP-MS分析顺铂及顺铂-BSA复合物铂,从而计算出顺铂和BSA的反应速率常数为0.077 h-1。方法对于铂的检出限为0.19 ng/g。 |
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| Bibliography: | As one of widely used anti-cancer drugs,cisplatin is administered intravenously in physiological saline.Therefore,it is very important to study the interaction between cisplatin and plasma proteins.Here we studied the reaction rate constant for cisplatin and bovine serum albumin（BSA） by size exclusion chromatography-inductively coupled plasma-mass spectrometry（SEC/ICP-MS）.Cisplatin was incubated with BSA at 37 ℃ for 3,6,9,17,and 24 h,respectively.The cisplatin and cisplatin-BSA adduct were analyzed by SEC/ICP-MS.The reaction rate constant for cisplatin and BSA is 0.077 h-1.The detection limit of platinum is 0.19 ng/g. 22-1125/O6 ZHAO Lei-Chao1,2,WANG Meng＊2,ZHENG Ling-Na2, GONG Xin2,WANG Bing2,FENG Wei-Yue2,LIANG Jin-Sheng1 1（School of Materials Science and Engineering,Hebei University of Technology,Tianjin 300130,China） 2（Key Laboratory for Bio-medical Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing |
| ISSN: | 0253-3820 |
| DOI: | 10.3724/SP.J.1096.2012.11344 |