Molecular cloning of novel cytochrome P450 1A genes from nine Japanese amphibian species

• Published in: Journal of Veterinary Medical Science Vol. 71; no. 10; pp. 1407 - 1411
• Main Authors: Nakayama, S.M.M., Hokkaido Univ., Sapporo (Japan), Tanaka Ueno, T, Sakamoto, K.Q, Fujita, S, Ishizuka, M
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.10.2009; Japan Science and Technology Agency
• Subjects: amphibian; AMPHIBIANS; Amphibians - genetics; Amphibians - metabolism; AMPHIBIEN; ANFIBIOS; Animals; Anura; Bufo japonicus; CITOCROMO P450; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; Cloning, Molecular; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; CYTOCHROME P450; cytochrome P450 1A; FILOGENIA; Freshwater; http://www.fao.org/aos/agrovoc#c_13325; http://www.fao.org/aos/agrovoc#c_27503; http://www.fao.org/aos/agrovoc#c_27583; http://www.fao.org/aos/agrovoc#c_32423; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_359; http://www.fao.org/aos/agrovoc#c_4039; Hyla japonica; JAPAN; JAPON; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PCR; phylogenetic tree; PHYLOGENIE; PHYLOGENY; Ranidae; Rhacophoridae; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; Urodela; Xenopus laevis; Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.001407

Novel cytochrome P450 1A (CYP1A) cDNA fragments were isolated from the liver of nine Japanese amphibian species using reverse transcription polymerase chain reaction (RT-PCR). Degenerate PCR primers were used to amplify 122-bp fragments of CYP1A cDNAs. Construction of a phylogenetic tree revealed that urodele and anuran amphibians formed two branches. Within the anuran species, three branches were formed: 1) Ranidae and Rhacophoridae, 2) Bufo japonicus formosus and Hyla japonica, and 3) Xenopus laevis. The cDNA nucleotide sequence of these CYP1A fragments showed identities ranging 72-98% (all), 72-78% (Anura vs. Urodela), 75 to 98% (Anura), 81% (Urodela), 74-80% (Xenopus laevis vs. nine Japanese amphibians).