Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content

• Published in: Journal of biotechnology Vol. 248; pp. 77 - 86
• Main Authors: Gouveia, Joao Diogo, Ruiz, Jesus, van den Broek, Lambertus A.M., Hesselink, Thamara, Peters, Sander, Kleinegris, Dorinde M.M., Smith, Alison G., van der Veen, Douwe, Barbosa, Maria J., Wijffels, Rene H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20.04.2017
• Subjects: Batch Cell Culture Techniques; BBP Biorefinery & Sustainable Value Chains; Biomass; Bioprocess Engineering; Bioprocestechnologie; BIOS Applied Bioinformatics; BIOS Applied Metabolic Systems; Botryococcus braunii; Carbohydrate; Chlorophyta - chemistry; Chlorophyta - classification; Chlorophyta - genetics; Chlorophyta - metabolism; DNA, Bacterial - analysis; DNA, Bacterial - genetics; FBR BP Biorefinery & Sustainable Value Chains; Fucose; Fucose - analysis; Fucose - chemistry; Fucose - metabolism; Galactose; Galactose - analysis; Galactose - chemistry; Galactose - metabolism; Hydrocarbons; Hydrocarbons - analysis; Hydrocarbons - chemistry; Hydrocarbons - metabolism; Leerstoelgroep Bioprocestechnologie; Microalgae; Photobioreactors; PRI BIOS Applied Bioinformatics; PRI BIOS Applied Metabolic Systems; RNA, Ribosomal, 18S - genetics; VLAG; Hydrocarbons; Carbohydrate; Microalgae; Galactose; Botryococcus braunii; Fucose
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2017.03.008

•16 commercial available strains compared under the same conditions.•High variability in carbohydrates and hydrocarbon content among the strains.•Glucose and galactose are the main monomeric saccharides for all strains.•Fucose highly present in CCALA778 strain.•K1489 strain is characterized for the first time. Botryococcus braunii can produce both long-chain hydrocarbons as well as carbohydrates in large quantities, and is therefore a promising industrial organism for the production of biopolymer building blocks. Many studies describe the use of different strains of Botryococcus braunii but differences in handling and cultivation conditions make the comparison between strains difficult. In this study, 16 B. braunii strains obtained from six culture collections were compared for their biomass productivity and hydrocarbon and carbohydrate content. Biomass productivity was highest for AC768 strain with 1.8gL−1day−1, while hydrocarbon production ranged from none to up to 42% per gram biomass dry weight, with Showa showing the highest hydrocarbon content followed by AC761. The total carbohydrate content varied from 20% to 76% per gram of the biomass dry weight, with CCALA777 as the highest producer. Glucose and galactose are the main monosaccharides in most strains and fucose content reached 463mgL−1 in CCALA778.