The metabolic effects of native and transgenic hemoglobins on plants

• Published in: Trends in biotechnology (Regular ed.) Vol. 17; no. 1; pp. 21 - 24
• Main Authors: Bülow, Leif, Holmberg, Niklas, Lilius, Gösta, Bailey, James E.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1999; Elsevier Science
• Subjects: anoxia; bacteria; Bacteria, Aerobic; Bacteria, Aerobic - metabolism; Biological and medical sciences; Bioprocess Technology; Bioprocessteknik; Biotechnology; Datura stramonium; Datura stramonium - metabolism; Engineering and Technology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; germination; Hemoglobins; Hemoglobins - genetics; Hemoglobins - metabolism; Industrial Biotechnology; Industriell bioteknik; Kinetics; Methods. Procedures. Technologies; Nicotiana - metabolism; Nicotine; Nicotine - biosynthesis; Oxygen; Oxygen - metabolism; plant physiology; Plants, Genetically Modified; Plants, Genetically Modified - metabolism; Plants, Medicinal; Plants, Toxic; Scopolamine - biosynthesis; Scopolamine Hydrobromide; Teknik; Tobacco; Transgenic animals and transgenic plants; Transgenic plants; Vitreoscilla; germination; Biotechnology; Vitreoscilla; anoxia; Plant biology; Biochemistry; tobacco; oxygen; Vitreoscillaceae; Recombinant microorganism; Alkaloid; Growth; Article; Hemoglobin; Bacteria; Transgenic plant; Mechanism of action; Secondary metabolism
• ISSN: 0167-7799; 1879-3096
• DOI: 10.1016/S0167-7799(98)01252-9

The strictly aerobic bacterium Vitreoscilla expresses a hemoglobin-like protein, VHb, when subjected to oxygen stress. When expressed in plants, this has several intriguing physiological effects, such as improving the overall growth rate, speeding germination and flowering, and increasing the productivity of certain oxygen-requiring metabolic pathways. Although the mechanisms behind the effects of VHb in heterologous hosts are not yet fully characterized, it has been suggested that VHb facilitates oxygen transport and/or storage. This hypothesis is supported by the kinetic properties of VHb, which allow very rapid dissociation of oxygen from the protein.