Exploring the arthritogenicity of Streptococcus dysgalactiae subspecies equisimilis

• Published in: BMC microbiology Vol. 18; no. 1; p. 17
• Main Authors: Oppegaard, Oddvar, Mylvaganam, Haima, Skrede, Steinar, Kittang, Bård Reiakvam
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27.02.2018; BioMed Central; BMC
• Subjects: Aged; Aged, 80 and over; Analysis; Antigens, Bacterial - genetics; Arthritis, Infectious - microbiology; Collagen; DNA, Bacterial - genetics; Female; Fibrinogen; Fibronectins; Fimbriae, Bacterial; Genetic Variation; Genotyping Techniques; Group C streptococcus; Group G streptococcus; Humans; Male; Middle Aged; Osteitis; Osteoarticular infections; Phylogeny; Septic arthritis; Streptococcal Infections - microbiology; Streptococcus; Streptococcus - classification; Streptococcus - genetics; Streptococcus - pathogenicity; Streptococcus dysgalactiae Subspecies equisimilis; Tissue tropism; Tropism; Whole Genome Sequencing; Streptococcus dysgalactiae Subspecies equisimilis; Group C streptococcus; Collagen; Tissue tropism; Osteoarticular infections; Septic arthritis; Pilus; Group G streptococcus; FCT-region; Fibronectin
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-018-1160-5

During the past decades, Streptococcus dysgalactiae subspecies equisimilis (SDSE) has been increasingly recognized as an important human pathogen. Osteoarticular infections is one of the predominant disease manifestations of SDSE, but the pathogenetic rationale for its arthritogenicity has yet to be unravelled. We aimed to explore if the rising incidence of osteoarticular infections caused by this pathogen in our region emanated from clonal expansion of strains with enhanced tropism for bone and joint tissue components or orthopaedic implants. Twenty-nine SDSE-isolates associated with osteoarticular infections were retrospectively identified. Their genomic content and affinity for fibronectin, collagen and stainless steel were compared to 24 temporally and geographically matched SDSE blood culture isolates obtained from patients without bone or joint infections. Despite a thorough genetic and phenotypic dissection, neither the presence or absence of any single gene, nor the binding abilities of the SDSE isolates, were predictive of clinical entity. SNP analysis revealed a heterogenous population, and a correlation between phylogenetic relationships and disease manifestation was not evident. However, we identified a strong concordance between phenotypic binding abilities and genetic variations in the pilus-region, also denoted as the FCT-region (Fibronectin binding, Collagen binding and T-antigen). This observation could be related to the ample and varied repertoire of putative adhesins residing within this region, including proteins predicted to adhere to fibronectin and collagen, as well as fibrinogen. SDSE strains associated with osteoarticular infections do not emanate from subpopulation characterized by distinct genetic or phenotypic traits. The genetic architecture of the pilus region was predictive of the adhesive properties of the SDSE-isolates, but its role in tissue tropism needs further investigation. To the best of our knowledge, this is the first comprehensive characterization of the genetic landscape of the SDSE pilus region.