A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus

The aberrant activity of CD4+ T cells in patients with systemic lupus erythematosus (SLE) is associated with DNA hypomethylation of the regulatory regions in CD11a and CD70 genes. Our previous studies demonstrated that Gadd45a contributes to the development of SLE by promoting DNA demethylation in C...

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Published inClinical & developmental immunology Vol. 2013; no. 2013; pp. 1 - 5
Main Authors Lu, Qianjin, Yung, Susan, Xiao, Rong, Liang, Gongping, Zhao, Ming, Huang, Chenghui, Li, Yaping, Chan, Tak Mao
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2013
Hindawi Limited
Wiley
Subjects
Adolescent
Adult
Autoimmune diseases
Case-Control Studies
CD11a antigen
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - metabolism
Cell Cycle Proteins - metabolism
DNA Methylation
Female
Gene Expression Regulation
HMGB1 Protein - genetics
HMGB1 Protein - metabolism
Humans
Immunology
Lupus
Lupus Erythematosus, Systemic - genetics
Lupus Erythematosus, Systemic - immunology
Lupus Erythematosus, Systemic - metabolism
Male
Middle Aged
Nuclear Proteins - metabolism
RNA, Messenger - genetics
Studies
Young Adult
China
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Summary:The aberrant activity of CD4+ T cells in patients with systemic lupus erythematosus (SLE) is associated with DNA hypomethylation of the regulatory regions in CD11a and CD70 genes. Our previous studies demonstrated that Gadd45a contributes to the development of SLE by promoting DNA demethylation in CD4+ T cells. In this study, we identified proteins that bind to Gadd45a in CD4+ T cells during SLE flare by using the method of co-immunoprecipitation and mass spectrometry, High mobility group box protein 1 (HMGB1) is one of identified proteins. Furthermore, gene and protein expression of HMGB1 was significantly increased in SLE CD4+ T cells compared to controls, and HMGB1 mRNA was correlated with CD11a and CD70 mRNA. A significant, positive correlation was found between HMGB1 mRNA and SLEDAI for SLE patients. Our data demonstrate that HMGB1 binds to Gadd45a and may be involved in DNA demethylation in CD4+ T cells during lupus flare.
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Academic Editor: Guixiu Shi
ISSN:2314-8861
1740-2522
2314-7156
1740-2530
DOI:10.1155/2013/206298