Identification of Anaplasma marginale long-term carrier cattle by detection of serum antibody to isolated MSP-3

• Published in: Journal of Clinical Microbiology Vol. 29; no. 4; pp. 788 - 793
• Main Authors: MCGUIRE, T. C, DAVIS, W. C, BRASSFIELD, A. L, MCELWAIN, T. F, PALMER, G. H
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.04.1991
• Subjects: anaplasma; Anaplasma - immunology; anaplasmose; anaplasmoses; anaplasmosis; Anaplasmosis - diagnosis; Animals; Antibodies, Monoclonal - immunology; anticorps monoclonal; anticuerpos monoclonales; antigene; antigenos; antigens; Antigens, Bacterial - immunology; Bacterial Outer Membrane Proteins - analysis; Bacterial Outer Membrane Proteins - immunology; Bacterial Proteins - immunology; Bacterial Vaccines; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; bovin; Carrier State - microbiology; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - microbiology; diagnosis; diagnostic; diagnostico; distribucion natural; distribution naturelle; epidemiologia; epidemiologie; epidemiology; Fundamental and applied biological sciences. Psychology; ganado bovino; immune serum; immunodiagnosis; immunodiagnostic; immunserum; inmunodiagnostico; Membrane Proteins - immunology; Microbiology; monoclonal antibodies; natural distribution; pathogenese; pathogenesis; patogenesis; Predictive Value of Tests; suero inmune; Immune response; Bovine; Antibody; Anaplasmataceae; Anaplasma marginale; Method; Cell surface; Rickettsiales; Proteins; Vertebrata; Mammalia; Immunoblotting assay; Bacteria; Serum; Artiodactyla; Detection; Ungulata; Humoral immunity
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.29.4.788-793.1991

Rapid and accurate detection of Anaplasma marginale-infected cattle would enhance anaplasmosis control procedures and evaluation of vaccines. Current tests based on detection of antibodies in serum are not widely used for several reasons, including the occurrence of either false-positive or false-negative results. We evaluated binding or antibodies in serum to a subunit antigen isolated from A. marginale initial bodies-major surface protein 3 (MSP-3). MSP-3 was detected in lysates of eight geographically different isolates of A. marginale and purified by affinity chromatography with monoclonal antibody AmG75C2. Antibodies from cattle infected with any of five geographically different isolates of A. marginale reacted in immunoblots with MSP-3. Sera from uninfected cattle and cattle infected with another rickettsial organism and two hemoprotozoal organisms failed to react with MSP-3. Six carrier cattle infected with the Florida isolate of A. marginale had antibody titers to MSP-3 ranging from 10(3) to 10(6) during a 5-year evaluation period. Since specific antibodies to isolated MSP-3 persist in high titers in long-term carrier cattle sera and MSP-3 is common among A. marginale isolates, it is recommended as a subunit antigen for an anaplasmosis test.