Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus

• Published in: Veterinary research (Paris) Vol. 48; no. 1; p. 41
• Main Authors: Wang, Chengbao, Meng, Han, Gao, Yujin, Gao, Hui, Guo, Kangkang, Almazan, Fernando, Sola, Isabel, Enjuanes, Luis, Zhang, Yanming, Abrahamyan, Levon
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.08.2017; BioMed Central; BMC
• Subjects: Cloning; Diseases; Gene expression; Gene Expression Regulation, Viral - genetics; Gene Expression Regulation, Viral - physiology; Genes, Viral - genetics; Genes, Viral - physiology; Genetic engineering; Genomes; Genotype & phenotype; Health aspects; Hogs; Life Sciences; Microscopy; Porcine respiratory and reproductive syndrome virus - genetics; Porcine respiratory and reproductive syndrome virus - physiology; Proteins; Short Report; Swine; Transcription factors; Transcription Factors - genetics; Transcription Factors - physiology; Virology; Virus Replication - genetics; Viruses
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-017-0445-2

In order to gain insight into the role of the transcription regulatory sequences (TRSs) in the regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus (PRRSV), the enhanced green fluorescent protein (EGFP) gene, under the control of the different structural gene TRSs, was inserted between the N gene and 3'-UTR of the PRRSV genome and EGFP expression was analyzed for each TRS. TRSs of all the studied structural genes of PRRSV positively modulated EGFP expression at different levels. Among the TRSs analyzed, those of GP2, GP5, M, and N genes highly enhanced EGFP expression without altering replication of PRRSV. These data indicated that structural gene TRSs could be an extremely useful tool for foreign gene expression using PRRSV as a vector.