Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types

• Published in: BioMed research international Vol. 2013; no. 2013; pp. 1 - 5
• Main Authors: Han, Kyou-Sup, Song, Junghan, Song, Eun Young, Kim, Taek Soo, Hong, Yun Ji, Chang, Ho Eun, Kim, Mi Jung, Hwang, Sang Mee, Park, Kyoung Un
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2013; Hindawi Limited
• Subjects: Antigens, CD - genetics; Antigens, Human Platelet - genetics; B-Lymphocytes - metabolism; Biomedical research; CD34 antigen; Embryonic Stem Cells - cytology; Fibroblasts - cytology; Fibroblasts - metabolism; Gene Expression Profiling; Gene Expression Regulation; Genotype; Genotype & phenotype; GPI-Linked Proteins - genetics; Granulocytes - metabolism; Hospitals; Humans; Immune system; K562 Cells; Killer Cells, Natural - metabolism; Leukemia; Lymphocytes; Medicine; Neoplasm Proteins - genetics; Population; Reproductive health; RNA, Messenger - genetics; Stem cells; Stem Cells - metabolism
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2013/946403

CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34− PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.