Comparison of the growth inhibitory effects of canine IFN-alpha, beta and -gamma on canine cells infected with Neospora caninum tachyzoites

• Published in: Journal of Veterinary Medical Science Vol. 63; no. 4; pp. 445 - 448
• Main Authors: Nishikawa, Y. (Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)), Iwata, A, Nagasawa, H, Fujisaki, K, Otsuka, H, Mikami, T
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.04.2001
• Subjects: Animals; Baculoviridae - chemistry; Baculoviridae - genetics; canine cell; Cell Survival - drug effects; CELLS; Cells, Cultured; Cloning, Molecular; Coccidiosis - drug therapy; Coccidiosis - veterinary; DNA - chemistry; Dog Diseases - parasitology; DOGS; Humans; interferon; INTERFERONS; Interferons - pharmacology; Kidney - cytology; Kidney - parasitology; Neospora - drug effects; Neospora - growth & development; NEOSPORA CANINUM; Recombinant Proteins - pharmacology; Reverse Transcriptase Polymerase Chain Reaction - veterinary
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.63.445

The growth inhibitory effects of recombinant canine interferon alpha (IFN-α), beta (IFN-β) and gamma (IFN-γ) were examined on Madin-Darby canine kidney cells infected with Neospora caninum tachyzoites. The parasite growth was inhibited by all IFNs in a dose-dependent manner. IFN-γ inhibited the parasite growth with greater efficacy than IFN-α or IFN-β. Moreover, the effect of IFNs on N. caninum growth associated with the suppression of the host cell viability. The present study indicates IFN-a and -β, besides IFN-γ, play a crucial role for N. caninum growth in host cells.