Overexpression of Bax inhibitor suppresses the fungal elicitor‐induced cell death in rice (Oryza sativa L.) cells

• Published in: The Plant journal : for cell and molecular biology Vol. 33; no. 3; pp. 425 - 434
• Main Authors: Matsumura, H., Nirasawa, S., Kiba, A., Urasaki, N., Saitoh, H., Ito, M., Kawai‐Yamada, M., Uchimiya, H., Terauchi, R.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.02.2003; Blackwell Science
• Subjects: Apoptosis - genetics; Apoptosis - physiology; Arabidopsis Proteins; Bax inhibitor; Biological and medical sciences; Carrier Proteins - genetics; Carrier Proteins - metabolism; cell death; Cells, Cultured; elicitor; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Gene Expression Regulation, Plant; Hydrogen Peroxide - metabolism; Hydrogen-Ion Concentration; Magnaporthe - growth & development; Magnaporthe grisea; Membrane Proteins - genetics; Membrane Proteins - metabolism; Oryza - genetics; Oryza - microbiology; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Leaves - genetics; Plant Leaves - microbiology; Plants, Genetically Modified; rice; SAGE; Cell culture; Monocotyledones; Plant pathogen; elicitor; SAGE; Alkalinization; rice; Genetic determinism; Hydrogen Peroxides; Cereal crop; Cell wall; Fungi; Oryza sativa; Regulation(control); Magnaporthe grisea; Bax inhibitor; Gramineae; Cell death; Angiospermae; Ascomycetes; Spermatophyta; Elicitation; Thallophyta
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.2003.01639.x

Summary Treatment of suspension‐cultured cells of rice (Oryza sativa L.) with cell wall extract of rice blast fungus (Magnaporthe grisea) elicits a rapid generation of H2O2, alkalinization of culture medium, and eventual cell death. To elucidate genes involved in these processes, we exploited SAGE (Serial Analysis of Gene Expression) technique for the molecular analysis of cell death in suspension‐cultured cells treated with the elicitor. Among the downregulated genes in the elicitor‐treated cells, a BI‐1 gene coding for Bax inhibitor was identified. Transgenic rice cells overexpressing Arabidopsis BI‐1 gene showed sustainable cell survival when challenged with M. grisea elicitor. Thus, the plant Bax inhibitor plays a functional role in regulating cell death in the rice cell culture system.