Genetic polymorphism of interleukin-17A and -17F genes in gastric carcinogenesis

• Published in: Human immunology Vol. 70; no. 7; pp. 547 - 551
• Main Authors: Shibata, Tomoyuki, Tahara, Tomomitsu, Hirata, Ichiro, Arisawa, Tomiyasu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2009
• Subjects: Adult; Aged; Allergy and Immunology; Analysis of Variance; Female; Gastric cancer; Gene Frequency; Genotype; Helicobacter Infections - complications; Humans; Inflammation; Interleukin-17 - genetics; Interleukin-17A; Interleukin-17F; Male; Middle Aged; PCR-SSCP; Polymerase Chain Reaction; Polymorphism; Polymorphism, Single Nucleotide; Polymorphism, Single-Stranded Conformational; Stomach Neoplasms - complications; Stomach Neoplasms - genetics; Interleukin-17F; Inflammation; Interleukin-17A; PCR-SSCP; Gastric cancer; Polymorphism
• ISSN: 0198-8859; 1879-1166; 1879-1166
• DOI: 10.1016/j.humimm.2009.04.030

Interleukin-17A (IL-17A) and IL-17F play a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the associations between gastric cancer and polymorphisms of IL-17A (rs2275913, G-197A) and -17F (rs763780, 7488 T/C) genes. The study was performed in 811 subjects (524 without gastric cancer and 287 with gastric cancer). We used the multiplex PCR-SSCP method to detect gene polymorphisms. Overall, the number of IL-17A/−197A allele was significantly correlated to the development of gastric cancer (OR, 1.42; 95% CI, 1.09–1.85; p = 0.010). The frequency of IL-17A/−197 A/A homozygote was also significantly higher in gastric cancer group than in non-cancer group (OR, 3.02; 95% CI, 1.86–4.91; p < 0.0001). The IL-17A/G-197A polymorphism was more closely correlated to intestinal-type cancer than diffuse-type cancer. In addition, IL-17A/−197A allele carriers had an increased risk of the development of gastric mucosal atrophy (OR, 1.68; 95% CI, 1.06–2.65; p = 0.026), and a positive relationship between the inflammation score and the number of −197A allele was observed ( p = 0.022). We concluded that G-197A polymorphism of IL-17A gene was significantly associated with the development of gastric cancer, especially intestinal-type cancer.