Removal of p-alkylphenols from aqueous solutions by combined use of mushroom tyrosinase and chitosan beads

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 10; pp. 2467 - 2475
• Main Authors: Yamada, K.(Nihon Univ., Narashino, Chiba (Japan). Coll. of Industrial Technology), Inoue, T, Akiba, Y, Kashiwada, A, Matsuda, K, Hirata, M
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.10.2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ADSORCION; ADSORPTION; Agaricales - enzymology; alkylphenol; BIODEGRADACION; BIODEGRADATION; Biological and medical sciences; CHAMPIGNON COMESTIBLE; CHITOSAN; CHITOSANE; CONTAMINANTES INDUSTRIALES; EDIBLE FUNGI; Endocrine Disruptors - metabolism; Environmental Pollutants - metabolism; Fundamental and applied biological sciences. Psychology; INDUSTRIAL POLLUTANTS; Monophenol Monooxygenase - metabolism; mushroom tyrosinase; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Phenols - metabolism; POLLUANT INDUSTRIEL; quinone formation; QUITOSANO; SETAS COMESTIBLES; Solutions; Water; Water Purification - methods; Enzyme; alkylphenol; Quinone; mushroom tyrosinase; Monophenol monooxygenase; Fungi; Adsorption; quinone formation; Phenols; Oxidoreductases; Chitosan; Aqueous solution; Cultivated mushroom; Thallophyta
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.60205

Eazymatic removal of p-alkylphenols from aqueous solutions was investigated through the two-step approach, the quinone conversion of p-alkylphenols with mushroom tyrosinase (EC 1.14.18.1) and the subsequent adsorption of quinone derivatives enzymatically generated on chitosan beads at pH 7.0 and 45 deg C as the optimum conditions. This technique is quite effective for removal of various p-alkylphenols from an aqueous solution. The % removal values of 97-100% were obtained for p-n-alkylphenols with carbon chain lengths of 5 to 9. In addition, removal of other p-alkylphenols was enhanced by increasing either the tyrosinase concentration or the amount of added chitosan beads, and their % removal values reached 93 except for 4-tert-pentylphenol. This technique was also applicable to remove 4-n-octylphenol (4NOP) and 4-n-nonylphenol (4NNP) as suspected endocrine disrupting chemicals. The reaction of quinone derivatives enzymatically generated with the chitosan's amino groups was confirmed by the appearance of peaks for UV-visible spectrum measurements of the chitosan films incubated in the p-alkylphenol and tyrosinase mixture solutions. In addition, 4-tert-pentylphenol underwent tyrosinase-catalyzed oxidation in the presence of hydrogen peroxide.