Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update

The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant re...

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Published inBMC veterinary research Vol. 12; no. 1; p. 166
Main Authors Rubio, Camila Peres, Hernández-Ruiz, Josefa, Martinez-Subiela, Silvia, Tvarijonaviciute, Asta, Ceron, José Joaquin
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 15.08.2016
BioMed Central
Subjects
Animals
antioxidant activity
Antioxidants
Antioxidants - analysis
Blood Chemical Analysis - methods
blood serum
Dogs
Dogs - blood
Health aspects
Humans
Inflammation
Review
Risk factors
Spectrophotometry
Spectrophotometry - veterinary
Veterinary Medicine - trends
Ferric
Human serum
Oxidative stress
Canine serum
Cupric
Trolox
Antioxidant activity
Online AccessGet full text
ISSN1746-6148
1746-6148
DOI10.1186/s12917-016-0792-7

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Abstract The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.
AbstractList The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.
The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.
ArticleNumber 166
Audience Academic
Author Hernández-Ruiz, Josefa
Ceron, José Joaquin
Rubio, Camila Peres
Tvarijonaviciute, Asta
Martinez-Subiela, Silvia
Author_xml – sequence: 1
  givenname: Camila Peres
  surname: Rubio
  fullname: Rubio, Camila Peres
– sequence: 2
  givenname: Josefa
  surname: Hernández-Ruiz
  fullname: Hernández-Ruiz, Josefa
– sequence: 3
  givenname: Silvia
  surname: Martinez-Subiela
  fullname: Martinez-Subiela, Silvia
– sequence: 4
  givenname: Asta
  surname: Tvarijonaviciute
  fullname: Tvarijonaviciute, Asta
– sequence: 5
  givenname: José Joaquin
  surname: Ceron
  fullname: Ceron, José Joaquin
BackLink https://www.ncbi.nlm.nih.gov/pubmed/27526688$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords Ferric
Human serum
Oxidative stress
Canine serum
Cupric
Trolox
Antioxidant activity
Language English
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Snippet The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total...
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StartPage 166
SubjectTerms Animals
antioxidant activity
Antioxidants
Antioxidants - analysis
Blood Chemical Analysis - methods
blood serum
Dogs
Dogs - blood
Health aspects
Humans
Inflammation
Review
Risk factors
Spectrophotometry
Spectrophotometry - veterinary
Veterinary Medicine - trends
Title Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update
URI https://www.ncbi.nlm.nih.gov/pubmed/27526688
https://www.proquest.com/docview/1812442087
https://www.proquest.com/docview/1846346614
https://pubmed.ncbi.nlm.nih.gov/PMC4986369
Volume 12
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