Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update

• Published in: BMC veterinary research Vol. 12; no. 1; p. 166
• Main Authors: Rubio, Camila Peres, Hernández-Ruiz, Josefa, Martinez-Subiela, Silvia, Tvarijonaviciute, Asta, Ceron, José Joaquin
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15.08.2016; BioMed Central
• Subjects: Animals; antioxidant activity; Antioxidants; Antioxidants - analysis; Blood Chemical Analysis - methods; blood serum; Dogs; Dogs - blood; Health aspects; Humans; Inflammation; Review; Risk factors; Spectrophotometry; Spectrophotometry - veterinary; Veterinary Medicine - trends; Ferric; Human serum; Oxidative stress; Canine serum; Cupric; Trolox; Antioxidant activity
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-016-0792-7

The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.