Vesicular storage of glycine in glutamatergic terminals in mouse hippocampus

• Published in: Neuroscience Vol. 242; pp. 110 - 127
• Main Authors: Muller, E, Bakkar, W, Martina, M, Sokolovski, A, Wong, A.Y.C, Legendre, P, Bergeron, R
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 09.07.2013; Elsevier; Elsevier - International Brain Research Organization
• Subjects: 4-AP; Animals; bafilomycin; Biological and medical sciences; Brain Stem - metabolism; CHO Cells; Cricetulus; Fundamental and applied biological sciences. Psychology; gamma-Aminobutyric Acid - metabolism; Glutamate Decarboxylase - metabolism; Glutamic Acid - metabolism; Glycine - metabolism; Glycine - physiology; Hippocampus - metabolism; Hippocampus - physiology; immunohistochemistry; Life Sciences; Membrane Potentials - physiology; Mice; Neurology; Neurons - metabolism; Neurons - physiology; Neurons and Cognition; Presynaptic Terminals - metabolism; Presynaptic Terminals - physiology; Receptors, Glycine - metabolism; Receptors, Glycine - physiology; Receptors, N-Methyl-D-Aspartate - metabolism; sniffer patch technique; synaptic transmission; Synaptic Transmission - physiology; Synaptic Vesicles - metabolism; Vertebrates: nervous system and sense organs; vesicular release; whole-cell patch-clamp; vesicular release; D(−)-2-amino-5-phosphonopentanoic acid; PFA; open probability; Chinese Hamster Ovary cell; MK-801; QX314; glycine receptor; gelatin in PBS; IPSC; synaptic transmission; paraformaldehyde; amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor; GlyR; NFPS; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; PBS; inhibitory postsynaptic current; D-AP-5; 1,2,3,4-tetrahydro-6-nitro-2,3-dioxobenzo-[ f]-quinoxaline-7-sulfonamide; sniffer patch technique; EGTA; NA; bafilomycin; whole-cell patch-clamp; NMDAR; SEM; standard error of the mean; ethylene glycol tetraacetic acid; glial fibrillary acidic protein; 4-aminopyridine; N-methyl-D-aspartate-sensitive glutamate; CHO cell; TTX; immunohistochemistry; WT; P open; N[3-(4-fluorophenil)-3-(4’-phenilphenoxy)]propylsarcosine; PBSG; GlyT1; phosphate buffer saline; ACSF; dizocilpine; lidocaine N-ethyl bromide; AMPAR; artificial cerebrospinal fluid; GFAP; 4-AP; glycine transporter type 1; HEPES; tetrodotoxin; wild-type; numerical aperture; NBQX; Popen; Immunohistochemistry; Rodentia; Central nervous system; Electrophysiology; Patch clamp method; Glycine; Encephalon; Vertebrata; Mammalia; Mouse; Synaptic transmission; Animal; Hippocampus; Release
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.03.007

Highlights • Immunocytochemical localization of glycine in CA1 pyramidal neurons. • Co-storage of glycine in glutamatergic presynaptic terminals. • Activity-dependent release of glycine. • Functional expression of synaptic and extrasynaptic GlyRs.