Exposure of Drosophila melanogaster to Mancozeb Induces Oxidative Damage and Modulates Nrf2 and HSP70/83

• Published in: Oxidative medicine and cellular longevity Vol. 2018; no. 2018; pp. 1 - 11
• Main Authors: de Carvalho, Nelson Rodrigues, Franco, Jeferson Luis, Posser, Thaís, Picoloto, Rochele Sogari, Nascimento, Mariele Samuel, Vieira, Patrícia de Brum, Rodrigues, Nathane Rosa, Echeverria Macedo, Giulianna, Felipe da Silva, Gustavo, da Rosa Ávila, Eduardo, Saraiva, Miriane Acosta, Kemmerich Martins, Illana
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2018; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Animals; Antioxidants; Behavior; Brain research; Diabetes; Drosophila; Drosophila melanogaster; Ethylene; Ethylenediaminetetraacetic acid; Fungicides, Industrial - adverse effects; Glutathione transferase; Health aspects; Heat; HSP70 Heat-Shock Proteins - metabolism; Insects; Lipid peroxidation; Mammals; Maneb - adverse effects; Mortality; NF-E2-Related Factor 2 - metabolism; Nitric oxide; Oxidative stress; Oxidative Stress - drug effects; Pharmacology; Proteins; Rats; Superoxide; Toxicology; Tropical diseases; Zineb - adverse effects; Brazil; United States > US
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2018/5456928

Mancozeb (MZ), a manganese- and zinc-containing ethylene-bis-dithiocarbamate, is a broad-spectrum fungicide. Harmful effects of this fungicide have been reported in nontarget organisms via a not fully understood mechanism. Drosophila melanogaster has provided remarkable contributions for toxicological studies. This work was aimed at evaluating the biochemical targets and implication of oxidative stress in MZ-mediated toxicity in drosophilas. Exposure of flies for fifteen days to MZ at 5 and 10 mg/mL through the diet impaired locomotor performance and induced fly mortality. In parallel, it caused lipid peroxidation and reactive oxygen species (ROS) formation and Mn overload. MZ inhibited superoxide dismutase and inducted catalase and glutathione S-transferase activities. Nitric oxide and reduced glutathione levels were significantly decreased by MZ. Heat shock proteins (HSP70 and HSP83) and Nrf2 mRNA levels were significantly augmented in MZ-exposed flies. Our study reinforced the use of Drosophila melanogaster as a reliable model for the study of biochemical targets of pesticides, and based on our data, MZ induced oxidative damage and Mn accumulation in a concentration-dependent manner. An adaptative cellular state was inducted by the lower concentration of pesticide, possibly contributing to the slighter damage observed.