Galangin Inhibits Cholangiocarcinoma Cell Growth and Metastasis through Downregulation of MicroRNA-21 Expression

• Published in: BioMed research international Vol. 2020; no. 2020; pp. 1 - 13
• Main Authors: Gong, Wencheng, Liu, Lijuan, Tou, Fangfang, Zhou, Min, Jiang, Li, Tong, Wei, Li, Wenqun, Zuo, Meiling, Kuang, Dabin, Li, Rong, Zou, Yang, Chen, Yin
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 2020; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: AKT protein; Anticancer properties; Antineoplastic Agents - pharmacology; Antitumor activity; Apoptosis; Apoptosis - drug effects; Bile Duct Neoplasms - metabolism; Biomedical research; Cancer; Care and treatment; Cell adhesion & migration; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement - drug effects; Cell proliferation; Cell Proliferation - drug effects; Cell viability; Cholangiocarcinoma; Cholangiocarcinoma - metabolism; Down-Regulation - drug effects; Flavonoids; Flavonoids - pharmacology; Homology; Humans; Kinases; Membranes; Metastases; Metastasis; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Phosphatases; Phosphorylation; Protein kinase; PTEN protein; Ribonucleic acid; RNA; Signal Transduction - drug effects; Software; Statistical analysis; Tensin; Wound healing; China; Japan
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2020/5846938

Galangin, a natural flavonoid product derived from the root of galangal, is emerging as a promising anticancer agent against multiple cancers. Yet, whether it also has antitumor effects on cholangiocarcinoma (CCA) and the underlying mechanism is still unknown. Herein, we demonstrate that galangin exhibits multiple antitumor effects on CCA cells including decreases cell viability; inhibits proliferation, migration, and invasion; and induces apoptosis. Moreover, those phenotypic changes are associated with downregulated microRNA-21 (miR-21) expression. To support, overexpression of miR-21 blocks galangin-mediated antisurvival and metastasis effects on CCA cells. Mechanically, galangin increases the expression of phosphatase and tensin homolog (PTEN), a direct target of miR-21, resulting in decreased phosphorylation of AKT, a protein kinase which plays a critical role in controlling survival and apoptosis. In contrast, overexpression of miR-21 abrogates galangin-regulated PTEN expression and AKT phosphorylation. Taken together, these findings indicate that galangin inhibits CCA cell proliferation and metastasis and induces cell apoptosis through a miR-21-dependent manner, and galangin may provide a novel potential therapeutic adjuvant to treat CCA.