Effect of Free Nitrous Acid on Nitrous Oxide Production and Denitrifying Phosphorus Removal by Polyphosphorus-Accumulating Organisms in Wastewater Treatment

• Published in: BioMed research international Vol. 2018; no. 2018; pp. 1 - 10
• Main Authors: Li, Aiguo, Wen, Xueyou, Fang, Xiaofeng, Zhao, Zhirui, Guo, Shan, Li, Duo, Miao, Zhijia, Wan, Jingmin
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2018; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Anesthetics; Bacteria - drug effects; Bacteria - metabolism; Batch Cell Culture Techniques; Bioaccumulation; Bioengineering; Bioreactors - microbiology; Biotechnology; Candidatus Accumulibacter phosphatis; Carbon; Denitrification - drug effects; Exhaustion; Knee; Laboratories; Metabolism; Nitrites; Nitrites - analysis; Nitrogen dioxide; Nitrous acid; Nitrous Acid - pharmacology; Nitrous oxide; Nitrous Oxide - metabolism; Oxidation-Reduction; pH effects; Phosphorus; Phosphorus - isolation & purification; Phosphorus metabolism; Phosphorus removal; Physiological aspects; Polyhydroxyalkanoates - metabolism; Production processes; Purification; Sewage; Temperature effects; Waste Water - microbiology; Wastewater treatment; Water Purification - methods; Water treatment
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2018/9192607

The inhibition of free nitrous acid (FNA) on denitrifying phosphorus removal has been widely reported for enhanced biological phosphorus removal; however, few studies focus on the nitrous oxide (N2O) production involved in this process. In this study, the effects of FNA on N2O production and anoxic phosphorus metabolism were investigated using phosphorus-accumulating organisms (PAOs) culture highly enriched (91±4%) in Candidatus Accumulibacter phosphatis. Results show that the FNA concentration notably inhibited anoxic phosphorus metabolism and phosphorus uptake. Poly-β-hydroxyalkanoate (PHA) degradation was completely inhibited when the FNA concentration was approximately 0.0923 mgHNO2-N/L. Higher initial FNA concentrations (0.00035 to 0.0103 mgHNO2-N/L) led to more PHA consumption/TN (0.444 to 0.916 mmol-C/(mmol-N·gVSS)). Moreover, it was found that FNA, rather than nitrite and pH, was likely the true inhibitor of N2O production. The highest proportion of N2O to TN was 78.42% at 0.0031 mgHNO2-N/L (equivalent to 42.44 mgNO2-N/L at pH 7.5), due to the simultaneous effects of FNA on the subsequent conversion of NO2 into N2O and then into N2. The traditional nitrite knee point can only indicate the exhaustion of nitrite, instead of the complete removal of TN.