Remote myocardial injury: the protective role of fluoxetine

• Published in: Canadian journal of physiology and pharmacology Vol. 96; no. 4; pp. 319 - 327
• Main Authors: Yaman, Onur M, Erman, Hayriye, Guner, Ibrahim, Tok, Olgu Enis, Pala, Mukaddes, Esrefoglu, Mukaddes, Gelisgen, Remise, Uzun, Hafize, Aksu, Ugur, Yelmen, Nermin, Sahin, Gulderen
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.04.2018; Canadian Science Publishing NRC Research Press
• Subjects: acute cardiac injury; Aorta; aorte abdominale infra-rénale; Creatine; Creatine kinase; Cytokines; Fluoxetine; fluoxétine; Heart; IL-1β; Inflammation; infrarenal abdominal aorta; Interleukin 10; Interleukin 6; Ischemia; ischemia–reperfusion; ischémie–reperfusion; Leukocytes; lésions cardiaques aiguës; Metastases; Myocardial diseases; oxidant/anti-oxidant balance; Oxidation; Oxidative stress; Pharmacology; Physiological aspects; Physiology; Reperfusion; stress oxydatif; Superoxide dismutase; Surgery; Thiobarbituric acid; Tumor necrosis factor-TNF; Tumor necrosis factor-α; équilibre oxydation/anti-oxydation; infrarenal abdominal aorta; oxidant/anti-oxidant balance; stress oxydatif; aorte abdominale infra-rénale; fluoxetine; fluoxétine; ischémie–reperfusion; inflammation; ischemia–reperfusion; équilibre oxydation/anti-oxydation; acute cardiac injury; lésions cardiaques aiguës; oxidative stress
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2017-0383

Aortic cross-clamping-induced ischemia–reperfusion (IR) is an important factor in the development of postoperative acute cardiac injury following abdominal aortic surgery. We investigated the possible anti-oxidant/anti-inflammatory effects of fluoxetine (FLX), which is used widely as a preoperative anxiolytic on cardiac injury induced by IR of the infrarenal abdominal aorta. FLX was administered to IR-performed (60 min of ischemia and 120 min of reperfusion) rats for 3 days, once daily at 20 mg/kg i.p. dosage. Results were compared to control and non-FLX-treated IR-performed rats. Serum creatine kinase (CK) and CK-MB levels, lipid hydroperoxide, thiobarbituric acid reactive substances, and pro-oxidant/anti-oxidant balance levels in the IR group were significantly higher whereas superoxide dismutase activity, glutathione, and ferric reducing/anti-oxidant power levels were lower than for the control. IR also increased myeloperoxidase activity, tumor necrosis factor-α, interleukin-1β, and interleukin-6 and decreased interleukin-10 levels. FLX decreased CK, CK-MB, lipid hydroperoxide, thiobarbituric acid reactive substances, and pro-oxidant/anti-oxidant balance levels while increasing superoxide dismutase activity, glutathione, and ferric reducing/anti-oxidant power levels. FLX also decreased myeloperoxidase activity, tumor necrosis factor-α, interleukin-1β, and interleukin-6 levels and increased interleukin-10 levels compared to IR. FLX attenuated the morphological changes associated with cardiac injury. Our study clearly demonstrates that FLX confers protection against aortic IR-induced cardiac injury, tissue leucocyte infiltration, and cellular integrity via its anti-oxidant/anti-inflammatory effects.