Bcl-xL overexpression protects from apoptosis induced by HMG-CoA reductase inhibitors in murine tubular cells

• Published in: Kidney international Vol. 64; no. 1; pp. 181 - 191
• Main Authors: Blanco-Colio, Luis Miguel, Justo, Pilar, Daehn, Ilse, Lorz, Corina, Ortiz, Alberto, Egido, Jesus
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.07.2003; Nature Publishing; Elsevier Limited
• Subjects: Acute-Phase Proteins - metabolism; Animals; apoptosis; Apoptosis - physiology; bcl-X Protein; Bcl-xL; Biological and medical sciences; Biological Transport - drug effects; Caspase 3; Caspase 9; Caspases - metabolism; Cells, Cultured; Cytochromes c - metabolism; Enzyme Activation; Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology; Kidney Tubules - cytology; Kidney Tubules - drug effects; Kidney Tubules - physiology; Medical sciences; Mice; Pharmacology. Drug treatments; Protein Prenylation - drug effects; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; RNA, Messenger - metabolism; Simvastatin - pharmacology; statins; tubular cells; Urinary system; apoptosis; Bcl-xL; tubular cells; statins; Kidney disease; Cell culture; Pathophysiology; Respiratory disease; Simvastatin; Rodentia; Statin derivative; Biological activity; Chemotherapy; Vertebrata; Renal tubule; Mammalia; Animal; Apoptosis
• ISSN: 0085-2538; 1523-1755
• DOI: 10.1046/j.1523-1755.2003.00080.x

Bcl-xL overexpression protects from apoptosis induced by HMG-CoA reductase inhibitors in murine tubular cells. Hyperplasia is attributed to enhanced tubular cell proliferation with unbalanced cell death. The 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors induce apoptosis in a variety of cell lines, including proximal tubular cells. However, the mechanisms by which statins induce apoptosis in tubular cells have not been fully addressed. Apoptosis induced by simvastatin was measured in murine tubular cells with and without overexpressing Bcl-xL. Expression of genes implicated in cell death was studied by Northern and Western blot. The treatment of proliferating murine tubular cells (MCT) with simvastatin induced apoptosis in a time- and dose-dependent manner (0.1 to 1 μmol/L). Apoptosis was correlated with Bcl-xL mRNA and protein down-regulation. By contrast, the treatment with simvastatin did not modify the expression of the proapoptotic protein Bax. Simvastatin treatment was associated with cytochrome C release from the mitochondria to the cytosol. We also observed the presence of active caspase 9 and 3 during apoptosis induced by simvastatin. These effects were reversed by mevalonate, farnesylpyrophosphate (FPP), and geranylgeranylpyrophosphate (GGPP), suggesting the involvement of protein prenylation. Simvastatin appears to alter the balance between cell-life and death-promoting genes, as reflected by the decreased Bcl-xL/Bax ratio. Supporting this hypothesis, overexpression of Bcl-xL reduced the amount of apoptosis induced by simvastatin by 80% when compared with control vector-expressing cells. The overexpression of Bcl-xL also prevented the activation of caspase 9 and 3. Our results indicate that down-regulation of Bcl-xL expression mediates apoptosis induced by statins in tubular cells. These results may be relevant to the treatment of disorders characterized by altered tubular proliferation.