Genotyping of clinical and environmental multidrug resistant Enterococcus faecium strains

• Published in: Indian journal of pathology & microbiology Vol. 60; no. 1; pp. 74 - 78
• Main Authors: Shokoohizadeh, Leili, Mobarez, Ashraf, Alebouyeh, Masoud, Zali, Mohammad, Ranjbar, Reza
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01.01.2017; Medknow Publications and Media Pvt. Ltd; Wolters Kluwer Medknow Publications
• Subjects: Cross Infection - microbiology; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-Field; Enterococcus; Enterococcus faecium; Enterococcus faecium - classification; Enterococcus faecium - drug effects; Enterococcus faecium - genetics; Enterococcus faecium - isolation & purification; Environmental Microbiology; Genetic aspects; Genetic Variation; Genotype; Genotypes; Gram-Positive Bacterial Infections - microbiology; Health aspects; Hospitals; Humans; Identification and classification; Intensive Care Units; Iran; Microbial drug resistance; multidrug resistant; Multilocus Sequence Typing; pulsed field gel electrophoresis; Virulence (Microbiology); Enterococcus faecium; multilocus sequence typing; pulsed field gel electrophoresis; multidrug resistant
• ISSN: 0377-4929; 0974-5130; 0974-5130
• DOI: 10.4103/0377-4929.200048

Context: Multidrug resistant (MDR) Enterococcus faecium is a nosocomial pathogen and clonal complex 17 (CC17) is the main genetic subpopulation of E. faecium in hospitals worldwide. Aims: There has thus far been no report of major E. faecium clones in Iranian hospitals. Subjects and Methods: The present study analyzed strains of MDR E. faecium obtained from patients and the Intensive Care Unit environments using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to determine the antibiotic resistance patterns and genetic features of the dominant. Results: clones of E. faecium. PFGE and MLST analysis revealed the presence of 17and 15 different subtypes, respectively. Of these, 18 (86%) isolates belonged toCC17. Most strains in this clonal complex harbored the esp gene and exhibited resistance to vancomycin, teicoplanin, ampicillin, ciprofloxacin, gentamicin, and erythromycin. The MLST results revealed 12 new sequence types (ST) for the first time. Approximately 50% of the STs were associated with ST203. Conclusion: Detection of E. faecium strains belonging to CC17 on medical equipment and in clinical specimens verified the circulation of high-risk MDR clones among the patients and in hospital environments in Iran.