Detection of Giardia duodenalis assemblage A and B isolates by immunochromatography in stool samples from Rwandan children

• Published in: Clinical microbiology and infection Vol. 20; no. 10; pp. O783 - O785
• Main Authors: Ignatius, R., Gahutu, J.B., Klotz, C., Musemakweri, A., Aebischer, T., Mockenhaupt, F.P.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2014; Elsevier Limited
• Subjects: Feces - parasitology; Genotypes; Giardia duodenalis; Giardia lamblia - classification; Giardia lamblia - isolation & purification; Giardiasis - diagnosis; Giardiasis - epidemiology; Humans; immunochromatography; Immunochromatography - methods; Microscopy; PCR; Polymerase Chain Reaction; Rwanda; Rwanda - epidemiology; Sensitivity and Specificity; Rwanda; immunochromatography; microscopy; Giardia duodenalis; Genotypes; PCR; Rwanda
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/1469-0691.12596

We evaluated the performance of an immunochromatographic assay (ICA) in comparison with light microscopy and PCR for the detection of Giardia duodenalis in stool samples from 558 Rwandan children. The association of infection with clinical symptoms was similar for the three diagnostic tools. The ICA equally detected parasites of assemblages A and B and was more sensitive than light microscopy (50.4 versus 29.5% of PCR-positive samples considered true positive; p <0.0001). Hence, the ICA shows superior sensitivity compared with microscopy but still misses half of the G. duodenalis infections detected by PCR in this hyperendemic area.