G-box motif (GCCACGTGCC) tetramer confers high-level constitutive expression in dicot and monocot plants

• Published in: The Plant journal : for cell and molecular biology Vol. 18; no. 4; pp. 443 - 448
• Main Authors: Ishige, F, Takaichi, M, Foster, R, Chua, N.H, Oeda, K
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.05.1999; Blackwell Science
• Subjects: beta-glucuronidase; Biological and medical sciences; buds; Cauliflower mosaic virus; Daucus carota; flowers; Fundamental and applied biological sciences. Psychology; gene expression; histochemistry; leaves; Molecular and cellular biology; Molecular genetics; Nicotiana tabacum; Oryza sativa; plant tissues; pollen; promoter regions; regulatory sequences; reporter genes; roots; seeds; Transcription. Transcription factor. Splicing. Rna processing; transgenic plants; Monocotyledones; Daucus carota; Transcription promoter; Tissue specificity; Gene expression; Nicotiana tabacum; Oryza sativa; Regulation(control); Gramineae; Dicotyledones; Angiospermae; Regulatory sequence; Spermatophyta; Solanaceae; Umbelliferae
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.1999.00456.x

GUS reporter expression from 11 basal promoters (CaMV-90) with G-box cores (CACGTG) was analysed to evaluate the regulatory roles of G-box flanking sequences. While most G-box motifs exhibited some tissue preference of gene expression, a distinct tissue-specific expression was not apparent. However, one of 11 G-box sequences, the G-box 10 (GCCACGTGCC) tetramer, conferred a high-level constitutive expression in seed, root, leaf, axillary bud, almost all parts of flower buds and pollen of transgenic tobacco plants. Furthermore, the G-box 10 tetramer promoter exhibited high-level expression in transgenic dicot carrot and monocot rice. This is apparently the first report of a G-box motif conferring a high-level constitutive expression in a non-tissue-specific manner.